PRIMER PICKING RESULTS FOR AT3_X1

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        279   19   60.60   57.89  2.00  1.00  7.00 CCCTGTGGAAGATTAGCGG
POSITION PENALTY, QUALITY: 0.000000, 1.599465
RIGHT PRIMER       361   19   60.58   57.89  6.00  0.00  9.00 GGGAAAGCTCACCCCTCTT
POSITION PENALTY, QUALITY: 0.000000, 1.580558
SEQUENCE SIZE: 641
INCLUDED REGION SIZE: 641

PRODUCT SIZE: 83, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,45

    0 TTCTATAAAGCTGAGGAGAAGAGTGAGGGAGTGTGGGCAAGAGAGGTGGCTCAGGCTTTC
                                                                  

   60 CCTGGGCCTGATTGAACTTTAAAACTTCTCTACTAATTAAACAACACTGGGCTCTACACT
                                                                  

  120 TTGCTTAACCCTGGGAACTGGTCATCAGCCTTTGACCTCAGTTCCCCCTCCTGACCAGCT
                                                                  

  180 CTCTGCCCCACCCTGTCCTCTGGAACCTCTGCGAGATTTAGAGGAAAGAACCAGTTTTCA
                                                                  

  240 GGCGGATTGCCTCAGATCACACTATCTCCACTTGCCCAGCCCTGTGGAAGATTAGCGGCC
                                             >>>>>>>>>>>>>>>>>>>**

  300 ATGTATTCCAATGTGATAGGAACTGTAACCTCTGGAAAAAGGTAAGAGGGGTGAGCTTTC
      *******************************************<<<<<<<<<<<<<<<<<

  360 CCCTTGCCTGCCCCTACTGGGTTTTGTGACCTCCAAAGGACTCACAGGAATGACCTCCAA
      <<                                                          

  420 CACCTTTGAGAAGACCAGGCCCTCTCCCTGGTAGTTACAGTCAAAGACCTGTTTGGAAGA
                                                                  

  480 CGTCATTTCAAGTGCTCTCCCTCCCACCCCACCTCTTGGGGTAAGGCCTTTCCTAAGCTA
                                                                  

  540 CCCCTTGGGTCCCTAGCCTAAGAAACAAGGGGGATGTCATCCCTGGTGTAAAGATGCTGT
                                                                  

  600 GCAGGAAGTCAGCACTCACGGGATCCAGGGGACGCTCCAAG
                                               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3504     0   289     0     0    16     0   818  1648     0     0     0     0     0   733
Right   3504     0   289     0     0     0     0   655  1723     0     0     0     0     0   837
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR AT3_X2

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   61.15   45.00  3.00  2.00 10.00 TTGGCATTTTGTCTCCTTGC
POSITION PENALTY, QUALITY: 0.000000, 1.148919
RIGHT PRIMER       688   19   60.79   57.89  5.00  3.00  9.00 GGCAGAAGACCTTTGGTCG
POSITION PENALTY, QUALITY: 20.000000, 21.785193
SEQUENCE SIZE: 967
INCLUDED REGION SIZE: 967

PRODUCT SIZE: 411, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,371

    0 AAACAGGAAGCTTCTCATTCTTTTTTCTCCCAGCGTGGGGAATTGAGCACATAATACTCC
                                                                  

   60 AAATAACCATCAGATGATTCTAATTCCAACATGACCACGTCCAGGCAACTGAACTGTCCC
                                                                  

  120 CTGGCAAGAAGTCTAGGACTGAACCTGTCCCGGGCCCCTGTACTTGGTTCAAAGGATTTA
                                                                  

  180 GCCTTTCTCTTGGCCACACCAGGTGGGCTGGAATCCTCTGCTTTACTGGGGCAACCCTGT
                                                                  

  240 GGTGGGCAGTGGGGCTAGGGGTTGCAGCCTAGCTTAACTTGGCATTTTGTCTCCTTGCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 GAAGGTTTATCTTTTGTCCTTGCTGCTCATTGGCTTCTGGGACTGCGTGACCTGTCACGG
      ************************************************************

  360 GAGCCCTGTGGACATCTGCACAGCCAAGCCGCGGGACATTCCCATGAATCCCATGTGCAT
      ************************************************************

  420 TTACCGCTCCCCGGAGAAGAAGGCAACTGAGGATGAGGGCTCAGAACAGAAGATCCCGGA
      ************************************************************

  480 GGCCACCAACCGGCGTGTCTGGGAACTGTCCAAGGCCAATTCCCGCTTTGCTACCACTTT
      ************************************************************

  540 CTATCAGCACCTGGCAGATTCCAAGAATGACAATGATAACATTTTCCTGTCACCCCTGAG
      ************************************************************

  600 TATCTCCACGGCTTTTGCTATGACCAAGCTGGGTGCCTGTAATGACACCCTCCAGCAACT
      ************************************************************

  660 GATGGAGGTACGACCAAAGGTCTTCTGCCCAGCCACCTTGTTAGGAGCACCTTTGGGCTT
      ********* <<<<<<<<<<<<<<<<<<<                               

  720 CCATAGGCCCAAGTCCAATGATTCCTCAACCAACACTGCAGCCACTAGGGGCGCTCATTA
                                                                  

  780 TGCATTACGATTCCCTTTGAACATCACTGTGTTATAATTCCCTTTGAAAATCATTTTTTA
                                                                  

  840 AAAAATTAGCCAAGGAATCTTGGCTATCTACTTTTTAAATCCTGGTTTCCTCTTTTGAGC
                                                                  

  900 ACCTTAAAATGGGGGAAGGCTTGTATCTTCTCTCAACTTCTTTTCAGTAATTCTTTCATC
                                                                  

  960 TATATGT
             

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    6612     0   289     0     0     1     0  1035  3921     0     0     0    22     0  1344
Right   6657     0   289     0     0   114     0  1327  3439     0     2     0    19     0  1467
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR AT3_X3

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        265   20   61.73   45.00  3.00  0.00 11.00 CCAAACCCACCACCATTTTT
POSITION PENALTY, QUALITY: 260.000000, 261.732281
RIGHT PRIMER       538   21   59.61   52.38  5.00  2.00  8.00 CAGGGGTAACATCTGCAACTC
POSITION PENALTY, QUALITY: 0.000000, 1.393460
SEQUENCE SIZE: 816
INCLUDED REGION SIZE: 816

PRODUCT SIZE: 274, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,220

    0 CAAGAGGACTTTTCCCATTCCAGCAAGAAAACGTCTGTGTGTTGATCTAGAGGCGTTTAG
                                                                  

   60 AGACTTTAGGTGGCAACCTAGTCTCTCTTTTTCCCTTTATCCTTCCTACCCTTCATTCTT
                                                                  

  120 CTTTTATCCTTTTATTCATCAGAACACAAGAGTTGAGCATTTATGCTGTCCCAGGTACTG
                                                                  

  180 TGCTTGAAGGAGTTAACAACTGAGGTGGCTATTAGTCAGAGACTGACCAGCATGTGCTCA
                                                                  

  240 CCACCCATGTTAACTAGGCAGCCCACCAAACCCACCACCATTTTTTTTTGACTTCTATAG
                               >>>>>>>>>>>>>>>>>>>>             **

  300 GTATTTAAGTTTGACACCATATCTGAGAAAACATCTGATCAGATCCACTTCTTCTTTGCC
      ************************************************************

  360 AAACTGAACTGCCGACTCTATCGAAAAGCCAACAAATCCTCCAAGTTAGTATCAGCCAAT
      ************************************************************

  420 CGCCTTTTTGGAGACAAATCCCTTACCTTCAATGAGACCTACCAGGACATCAGTGAGTTG
      ************************************************************

  480 GTATATGGAGCCAAGCTCCAGCCCCTGGACTTCAAGGTGAGTTGCAGATGTTACCCCTGA
      **************************************<<<<<<<<<<<<<<<<<<<<< 

  540 CCTCCGAGTTCTTCCTCTCCACTCAGAGATTGAGGAGGTGGAGAAACAGCATCCAAATTC
                                                                  

  600 ACACTGCTTTGCTGCTGAAGACTGCTGGAGGGCTGACTAAAAGTTAGAACCCCTGCAATA
                                                                  

  660 GTTATTCTTACTTGAAACCTGAGAAATCAAAGGTATCCATGCTTGGATTGTAGTGACTGC
                                                                  

  720 CCAGAAAACATGAATTAATAATCAATTCTTCATTCCATCCACCAACTTCAAATATATACC
                                                                  

  780 AAAGGGTGTTTTGAAGATGCCAGTTCTACAAGATAT
                                          

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    5081     0   289     0     0    13     0  1874  1352     0     0     0    25     0  1528
Right   5254     0   289     0     0   103     0  1706  1557     0     0     0     0     0  1599
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR AT3_X3'

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        265   27   60.24   37.04  4.00  0.00 11.00 CACAGGTGAGTAGGTTTATTTTCTGTT
POSITION PENALTY, QUALITY: 120.000000, 127.241506
RIGHT PRIMER       458   19   60.21   57.89  4.00  3.00  9.00 CTCCAGGGCCATTCTGAGT
POSITION PENALTY, QUALITY: 0.000000, 1.206859
SEQUENCE SIZE: 738
INCLUDED REGION SIZE: 738

PRODUCT SIZE: 194, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,142

    0 AGTCAGAGAAAATAATATTTTATCTTCTTTTTCCCAGACTATTTTCGCCTTCCTTCTTTT
                                                                  

   60 CATTTATTTCTTCCTATTTCTTTTTGTCTTTTTCTTCTGATAATATTTATTAACTACAGG
                                                                  

  120 AAAGATTCATGGAACTATATTAGATATGTGAGGCTTCCCAATTTGGGTTAGAGCAATGGC
                                                                  

  180 TTCTTAATCAAATGGTGGGAAAGGACAGAGGGATGGTGAGAAAAATAAAATGCTGCCTGG
                                                                  

  240 GAAAATGGAGAAGCCAATTGAATAGCACAGGTGAGTAGGTTTATTTTCTGTTCTCCTCAG
                               >>>>>>>>>>>>>>>>>>>>>>>>>>>      **

  300 GAAAATGCAGAGCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGC
      ************************************************************

  360 CGAATCACCGATGTCATTCCCTCGGAAGCCATCAATGAGCTCACTGTTCTGGTGCTGGTT
      ************************************************************

  420 AACACCATTTACTTCAAGGTACTCAGAATGGCCCTGGAGAGACCCCAGGGACTTCCTCTT
      ********************<<<<<<<<<<<<<<<<<<<                     

  480 GCTCTTCAGCTTACCCCCTTTTTTTTTAAATGGCGAGACCGAAGCCCTGAGAGGGCAAAT
                                                                  

  540 GGACTGCCGAAAGCTACACAGGTACAGGTCAGCAGGGCAGGTCAATCTATTATTTATTTA
                                                                  

  600 TTTATTTATTTTTGACAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATC
                                                                  

  660 TCGGCTCACTGCAAGCTTCGCCTCCTGGGTTCTCGGCATTCTCCTGCCTCAGCCTCCCAA
                                                                  

  720 GTAGCTGGGAATACAGGC
                        

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4474     0   289     0     0   273     0  1464  1424     0     0    13     0     0  1011
Right   4294     0   289     0     0   209     0   512  2472     0     0     0    25     0   787
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR AT3_X4

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        273   25   58.09   36.00  3.00  0.00 10.00 TGTTCTTACTTTGTGATTCTCTTCC
POSITION PENALTY, QUALITY: 0.000000, 6.906112
RIGHT PRIMER       715   23   60.12   47.83  5.00  2.00 11.00 GGAGGAAACTCCTTCCTAGACAA
POSITION PENALTY, QUALITY: 0.000000, 3.115306
SEQUENCE SIZE: 991
INCLUDED REGION SIZE: 991

PRODUCT SIZE: 443, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,395

    0 GGCTTTGTCACTCATTTCTTCTTAGCATGGAATAGCATTACATTTGGTCTGGATGTACCA
                                                                  

   60 CAGTCTGTCTATTCATCTACTGAAGGACATTTTGGCTGCTTCCAAGGTTTGACAGTTATG
                                                                  

  120 AATAAACCTACTCATAATTCCATCATTCTGACACAGCCATTGTTAACCTTTTTGTGCATA
                                                                  

  180 TCCCGCCAGTCTTTTTTCCGAATAATTATATATTAATGTAACACTATAATATGGATATGT
                                                                  

  240 CTGTGTCAATAACTATCCTCCTATGAATGTTTGTGTTCTTACTTTGTGATTCTCTTCCAG
                                       >>>>>>>>>>>>>>>>>>>>>>>>>**

  300 GGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGGAACTGTTCTACAAGGCT
      ************************************************************

  360 GATGGAGAGTCGTGTTCAGCATCTATGATGTACCAGGAAGGCAAGTTCCGTTATCGGCGC
      ************************************************************

  420 GTGGCTGAAGGCACCCAGGTGCTTGAGTTGCCCTTCAAAGGTGATGACATCACCATGGTC
      ************************************************************

  480 CTCATCTTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGAAGGAACTCACCCCAGAG
      ************************************************************

  540 GTGCTGCAGGAGTGGCTGGATGAATTGGAGGAGATGATGCTGGTGGTCCACATGCCCCGC
      ************************************************************

  600 TTCCGCATTGAGGACGGCTTCAGTTTGAAGGAGCAGCTGCAAGACATGGGCCTTGTCGAT
      ************************************************************

  660 CTGTTCAGCCCTGAAAAGTCCAAACTCCCAGGTTTGTCTAGGAAGGAGTTTCCTCCCTTC
      *********************************<<<<<<<<<<<<<<<<<<<<<<<    

  720 TCCACCCGCAAGGTAGTCTGACCAAAAGTGGAAGAGTTGGAGAAAGAATAGAAAGGAGCA
                                                                  

  780 ACAAGTCAGGACTCCTGGATACTGATCCTAGTTTCTACTGCTAATTTGTGGAAATCTCTT
                                                                  

  840 TTCCTTTTGAGACCTCAGTTTCCTCTTCTGTAAAAGGGAAGTTTGTTCTTGGATCTCCAT
                                                                  

  900 GGGCCCAGCCAGCACTGGTGCCCTGTGAGTCTGTATCAGGTAGAGGAGATGGGACCAGGT
                                                                  

  960 GGAGAGGAATTTGAAAGGGCATTGGAATTCA
                                     

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    6872     0   289     0     0   226     0  1924  2932     0     0     0    17     0  1484
Right   7004     0   289     0     0     0     0  1493  3474     0     0     0     0     0  1748
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR AT3_X5

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        276   21   58.94   52.38  4.00  0.00  8.00 AGACTTCTCCGGTCTTCCTTC
POSITION PENALTY, QUALITY: 20.000000, 22.061137
RIGHT PRIMER       389   22   58.28   50.00  4.00  2.00 11.00 AAGAGAGTGGGGAAGGTGTACT
POSITION PENALTY, QUALITY: 20.000000, 23.722528
SEQUENCE SIZE: 665
INCLUDED REGION SIZE: 665

PRODUCT SIZE: 114, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,69

    0 TAGAGACAAGGTTTCACCATATTAGTTAGAGTGTCTCCAACTCCTGACCTCAGGTGATCT
                                                                  

   60 GTACACCTTGGCCTCCCGAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCAGCCCCC
                                                                  

  120 AAAGTACTTTATTATTTTTAACACATATTCATTGTGAGAGTATGATTAGGTGAAGATTTA
                                                                  

  180 GGATTTCTTCTTATGTTTCAAAAAGCCCCAAAGGATCTCTTAATCCAAACTGAATTCCCA
                                                                  

  240 TCTGTGGATTGAAGCCAACTTTCTCCCATCTCACAAAGACTTCTCCGGTCTTCCTTCCAG
                                          >>>>>>>>>>>>>>>>>>>>> **

  300 GTATTGTTGCAGAAGGCCGAGATGACCTCTATGTCTCAGATGCATTCCATAAGGCATTTC
      ************************************************************

  360 TTGAGGTGAGTACACCTTCCCCACTCTCTTAGGGTACAGAAAGGAGATGCATGAACAGCA
      ******* <<<<<<<<<<<<<<<<<<<<<<                              

  420 GGAACACGTGGAAAAGGCCTGTTTCCAGTGTTAAGGCATGCAAAAGGCCTCCACAGGCTG
                                                                  

  480 CTATAATACAGCCCTCTCCAAAACCTTCATGGTGTGATTGTTCTGCCTTCCCTCCCACTA
                                                                  

  540 CCTCTTCTGTAGCAGGTCAAGCGGGAACACAAACATTTAGGGAGGGTGATATAGGAAAAG
                                                                  

  600 AAGCCAGCAAAGGCCATCAAGAAGAAATTTACAGCATGAGGAGAACCAGAAGAGTATGGG
                                                                  

  660 GTCGC
           

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3744     0   289     0     0   148     0  1249  1208     0     0     0     0     0   850
Right   3744     0   289     0     0     0     0   874  1504     0     8     0     0     0  1069
Pair Stats:
considered 21, high end compl 20, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR AT3_X6

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   58.42   45.00  6.00  3.00  8.00 TTTATTCCCATGTGACCTGC
POSITION PENALTY, QUALITY: 0.000000, 1.577239
RIGHT PRIMER       503   22   57.63   40.91  4.00  0.00 11.00 GGAAGAGGTGCAAAGAATAAGA
POSITION PENALTY, QUALITY: 60.000000, 64.365428
SEQUENCE SIZE: 732
INCLUDED REGION SIZE: 732

PRODUCT SIZE: 226, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,181

    0 CCATTTACAATGGTACCAGCCAGAAATGTGATAACCTGTGTACATTCAGATTTCTGGGTT
                                                                  

   60 ACCTGAATGGAACTCTTACACTTATTACCTAGCACAAGGCTTGGACAAACACAAGTACCT
                                                                  

  120 TACATTCTCTGCATGAAAGAATGAGTGAAAGTAGGATTCTGGAGGGAATCCAACCTGACC
                                                                  

  180 CAAATGTACTTTTTACTGGAAAACAAAAGCATTTGAGGAATTGCTGTGTCTGTGGATGAT
                                                                  

  240 TTACCTGCCAAAATGAACGGCAGAGTGGCTAATTTAGTTTTATTCCCATGTGACCTGCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 GTAAATGAAGAAGGCAGTGAAGCAGCTGCAAGTACCGCTGTTGTGATTGCTGGCCGTTCG
      ************************************************************

  360 CTAAACCCCAACAGGGTGACTTTCAAGGCCAACAGGCCTTTCCTGGTTTTTATAAGAGAA
      ************************************************************

  420 GTTCCTCTGAACACTATTATCTTCATGGGCAGAGTAGCCAACCCTTGTGTTAAGTAAAAT
      *********************************************************** 

  480 GTTCTTATTCTTTGCACCTCTTCCTATTTTTGGTTTGTGAACAGAAGTAAAAATAAATAC
        <<<<<<<<<<<<<<<<<<<<<<                                    

  540 AAACTACTTCCATCTCACATTATAAATGGACTCTGCATTTGAAATGAAGACAAGGAAAGG
                                                                  

  600 GGAAACATGCTATTGGGGCACATGGTAAAATGATGCCTTCAAGTTGTTCTTTACCCAGTA
                                                                  

  660 ACCACATCTGGATCAAGAAAATGAGGGAGAGAGCGATAAAAGATGGTAGACAGCCAGAAA
                                                                  

  720 GGGAAGGGAGAG
                  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4819     0   244     0     0     0     0  1696  1512     0     0     0     0     0  1367
Right   4414     0   289     0     0   103     0  1578  1288     0     0    14     0     0  1142
Pair Stats:
considered 3, high end compl 2, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X1

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        276   22   57.84   45.45  3.00  2.00 10.00 CTTACACACCACTGCCTGATAA
POSITION PENALTY, QUALITY: 0.000000, 4.158608
RIGHT PRIMER       442   23   60.70   47.83  6.00  2.00  9.00 GGCAGACAGATGCACTGATACTT
POSITION PENALTY, QUALITY: 0.000000, 3.704304
SEQUENCE SIZE: 718
INCLUDED REGION SIZE: 718

PRODUCT SIZE: 167, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,122

    0 AGTAAAAATAAATGAATTCATGGAAACATATTAAGCAATTATCCAGATAACATAAGGGAT
                                                                  

   60 GGCAAAAATGGTGCAGATGGTGGAGGGGAGACAAGTAGAAGTTGGGGTGCTCTTGTTGAA
                                                                  

  120 TGTCTGGCTCTGAACTCTAGAGGAGGCCGCAGGGGCTGGGCAGGAAGGAGGTGAATCTCT
                                                                  

  180 GGGGCCAGGAAGACCCTGCTGCCCGGAAGAGCCTCATGTTCCGTGGGGGCTGGGCGGACA
                                                                  

  240 TACATATACGGGCTCCAGGCTGAACGGCTCGGGCCACTTACACACCACTGCCTGATAACC
                                          >>>>>>>>>>>>>>>>>>>>>>**

  300 ATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAA
      ************************************************************

  360 GGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTGT
      ************************************************************

  420 AAGTATCAGTGCATCTGTCTGCCCTGCCAGGGGTCTTTTCATGGACACCCACTATGCCAG
      <<<<<<<<<<<<<<<<<<<<<<<                                     

  480 GAGCCTCCCTGGCCTGAAGCCAGCCCTGAAGCCGGCTGCCACACTAGCCCAGAGAGAGGA
                                                                  

  540 GTGCCCTGGGAGGGAGATGGGCTGAGTGGAGCTGTCATCACCCCCTCCTGACCTCGCCTT
                                                                  

  600 CAAGGTCAAGTTCTTTGGTGAGAAGGTCCTAGCTGCATTGCAAACAGCCAGGTATAGGGA
                                                                  

  660 TTTGTGTTTGTCTGCGACCCAGAATCACTGGGGTTCGAGTTAGGGTTCAGATCTGAGC
                                                                

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4274     0   289     0     0    68     0   569  2779     0     0     0     0     0   569
Right   4274     0   289     0     0     0     0   375  2978     0     0     0     0     0   632
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X2

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        280   18   59.59   61.11  3.00  0.00  8.00 ATCCACTGCCCTCCCTCT
POSITION PENALTY, QUALITY: 0.000000, 2.407131
RIGHT PRIMER       443   18   62.34   66.67  3.00  2.00  8.00 AGCCAGGTCACACCCGAG
POSITION PENALTY, QUALITY: 180.000000, 184.341886
SEQUENCE SIZE: 549
INCLUDED REGION SIZE: 549

PRODUCT SIZE: 164, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,119

    0 CTAGGGTTAGTGTGCGGCTGGGCTCAGGGCTGCCCCATTTGCTAGGATCGTGGGGTTCCC
                                                                  

   60 ATGTGTCAGGATCCAGAGGCTAGGGTATGATCAGGATCTCTAGCTGGGGTCAGGGTCAGA
                                                                  

  120 GCTCTCTGTGTCCCCTAGAATTGCCATCAACCTTAAACCCAGAGGAGGCCCAGTCCAACC
                                                                  

  180 CCTCAGCTTTAAGACCTGGGAGCCTCATCTCAGAGAGGCTGAGTCATGGCCAAGGCAGTT
                                                                  

  240 GGGGTGGGAGCAGGGGGCTTGGTGTGGGCCTGCAGCCCTCATCCACTGCCCTCCCTCTAG
                                              >>>>>>>>>>>>>>>>>>**

  300 TGAACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATA
      ************************************************************

  360 TCACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAAGTGAG
      *********************************************************   

  420 TCGGGCCTCGGGTGTGACCTGGCTGGGGGTAGGGTGGCGGGAGGAACAGCCTGGGCTTCC
            <<<<<<<<<<<<<<<<<<                                    

  480 CCCAGCCACAGGGAGGAAAGGCAGCAGCTGGGGGACTCAGGTCTCTCCCCTTGATTTGGA
                                                                  

  540 ACCAGAGCC
               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4078     0   123     0     0     7     0   374  2820     0     0     0     0     0   754
Right   2584     0   289     0     0     1     0    95  1901     0     0     0     0     0   298
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X3

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   60.82   55.00  2.00  0.00  9.00 CCTCACTTCCATTCCTTCCC
POSITION PENALTY, QUALITY: 0.000000, 0.821928
RIGHT PRIMER       457   21   60.41   47.62  8.00  2.00  9.00 CATCAGCTGACAGGAATGCTT
POSITION PENALTY, QUALITY: 0.000000, 1.412222
SEQUENCE SIZE: 735
INCLUDED REGION SIZE: 735

PRODUCT SIZE: 180, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,139

    0 TGCCTCCTCTTCCACTTTTCGGTACCCTGTGATTGATTGGGACCACCCAGATAACCTAGG
                                                                  

   60 ATCATCTCCCCACCTACCCCAAGGTCCTTAACTTAACCATACTTCATATGGGTAACACGA
                                                                  

  120 GTTGAGTGTGGTACCCAGGTTTGACATGTTGGGTAACATATTTGCAGGTTCTGTGGATTA
                                                                  

  180 GGAGGACATTTTGGGGGCCATGATTCTATCTTCCACCCTCGCCTAGACAAAATTGGAGGC
                                                                  

  240 TCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTCACTTCCATTCCTTCCCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 CATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTC
      ************************************************************

  360 CATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTA
      ************************************************************

  420 CACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCCATGCCCTGGCCCTCTCTG
      *****************<<<<<<<<<<<<<<<<<<<<<                      

  480 GGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAGGCTGGGTATTGATCAGTC
                                                                  

  540 CATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAACACACAGCTGAGTATGTG
                                                                  

  600 TCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTTGGCTCAGCAGAGGGGGAG
                                                                  

  660 GTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGCCTGGGAAGTTTGCAGGGT
                                                                  

  720 TGGGGACCCCAGAGC
                     

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4444     0   289     0     0     0     0   844  2220     0     0     0     0     0  1091
Right   4444     0   289     0     0     0     0   400  2950     0     0     0     0     0   805
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X4

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        280   18   59.59   61.11  3.00  0.00  9.00 GGGTCCTTGGCTCTTTCC
POSITION PENALTY, QUALITY: 0.000000, 2.413192
RIGHT PRIMER       392   20   61.28   55.00  3.00  2.00  9.00 CCAGAGGACGCTTGACACAT
POSITION PENALTY, QUALITY: 0.000000, 1.279241
SEQUENCE SIZE: 671
INCLUDED REGION SIZE: 671

PRODUCT SIZE: 113, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,75

    0 CTAGACAAAATTGGAGGCTCACTCCTTGGGCTCCCTGGATGACCCCCAACATCCTTCCTC
                                                                  

   60 ACTTCCATTCCTTCCCAGCATCCAGATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGA
                                                                  

  120 GCTGGTGGAAGCCAAGTCCATTGATGTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGG
                                                                  

  180 GACCCTGAAGTATGGCTACACCACTGCCTGGTGGTAAGCATTCCTGTCAGCTGATGCCCC
                                                                  

  240 ATGCCCTGGCCCTCTCTGGGTGGAGGGCTGAATGAGGTCTGGGTCCTTGGCTCTTTCCAG
                                              >>>>>>>>>>>>>>>>>>**

  300 GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAA
      ************************************************************

  360 CACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTT
      *************<<<<<<<<<<<<<<<<<<<<                           

  420 GGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGC
                                                                  

  480 CTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCA
                                                                  

  540 GCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAG
                                                                  

  600 CCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGC
                                                                  

  660 TGCTCCTGCAT
                 

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3804     0   289     0     0     0     0   382  2360     0     0     0     0     0   773
Right   3804     0   289     0     0     0     0   348  2565     0     0     0     0     0   602
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X5

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        280   18   62.73   66.67  4.00  2.00  8.00 CATGTCGGGTCTCCCTGC
POSITION PENALTY, QUALITY: 0.000000, 4.729227
RIGHT PRIMER       409   20   62.18   60.00  4.00  0.00  8.00 GGGCACAGGGTGGTGTACTT
POSITION PENALTY, QUALITY: 0.000000, 2.179675
SEQUENCE SIZE: 688
INCLUDED REGION SIZE: 688

PRODUCT SIZE: 130, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,92

    0 GCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTCCAGATCAA
                                                                  

   60 CACACAGCTGAGTATGTGTCAAGCGTCCTCTGGGGAAGTGGGAGCTGGACTCCAGGGCTT
                                                                  

  120 GGCTCAGCAGAGGGGGAGGTTGTGCAGGCAGAGGGTTCTGGGGCCACCAAAGGAGGCAGC
                                                                  

  180 CTGGGAAGTTTGCAGGGTTGGGGACCCCAGAGCTGGCCAAGCTCTTGACTGGCCTGGGCA
                                                                  

  240 GCATGTGGATACCATCTGATAGCGGAGGCTGCCCTGAGGTCATGTCGGGTCTCCCTGCAG
                                              >>>>>>>>>>>>>>>>>>**

  300 CCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGCTACCTGTCTTTCCATAAGC
      ************************************************************

  360 TGCTCCTGCATCTCCAAGGGGAGCGAGAGTAAGTACACCACCCTGTGCCCCCATTCCTGT
      ******************************<<<<<<<<<<<<<<<<<<<<          

  420 CGTGCCCATCCTGTTAGTGTGTCCACGGCCCCCTCCAGGCTCAACCCCACACAGGGATGC
                                                                  

  480 TTGTGGGTGGCCAAACCTGAGGGCAGCAATACCTTCAGTGGGGTCATTCCATCCCCCTCC
                                                                  

  540 ATCAATACACCCTAAAGGCTGGAAACAACAATAACCAACAGCTAGTAACTAACAGCTATT
                                                                  

  600 AAGAACTTCTGTTGGCAAAGCACTATTCCAAGCCCTTTCATGAATTAATTGATTTTGTCC
                                                                  

  660 TTAAAACCAACCCTAGGATATAGATTCT
                                  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3974     0   289     0     0     0     0   348  2707     0     0     0     0     0   630
Right   3974     0   289     0     0    34     0   889  1971     0     0     0     0     0   791
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X6

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        276   18   59.99   55.56  3.00  3.00  9.00 CATTGTGGTGCTTGCTGC
POSITION PENALTY, QUALITY: 80.000000, 82.014189
RIGHT PRIMER       395   19   62.38   63.16  3.00  3.00  9.00 ACAGGGAGTCAGCCAGCCT
POSITION PENALTY, QUALITY: 100.000000, 103.379680
SEQUENCE SIZE: 670
INCLUDED REGION SIZE: 670

PRODUCT SIZE: 120, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,74

    0 TTTCTGACTCTGGAGCAAGACGGATACATGTATGAATTTGGACTCTAGACACGTTCTCGT
                                                                  

   60 GTGTGTGACAGGTGTGAGCGTCACAGGAGCTGGGCCCTCCCGAGGAATTCTGGATGGTGC
                                                                  

  120 CACAGTTAATTCTTGGGTCTGAGGCTCCGTGTTCTCACTGCAAAATGGGAGTGATAATTC
                                                                  

  180 TTACTTCCTGAGCTACAAGAGTCAGGGCCAACAGAGCCATGAAGGAGCCTGGTACACACT
                                                                  

  240 AGGCGCTCCATGGATGCACAGGACTGGTCAGGGGCTCATTGTGGTGCTTGCTGCCTTCAG
                                          >>>>>>>>>>>>>>>>>>    **

  300 GCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCT
      ************************************************************

  360 GAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCATGCCCAGGAGGCT
      ************     <<<<<<<<<<<<<<<<<<<                        

  420 GGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACCTTCTCCATGTGGC
                                                                  

  480 CAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGAAGGAGGCACTCCG
                                                                  

  540 TCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAGATCTGCAAAGAGA
                                                                  

  600 TCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGGTGAGTGCGTTT
                                                                  

  660 CTGTCTGCAT
                

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3794     0   289     0     0     0     0   681  1966     0     0     0     0     0   858
Right   3794     0   289     0     0     0     0   274  2616     2     3     0     0     0   610
Pair Stats:
considered 40, high end compl 38, ok 2
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X7

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        271   20   60.59   55.00  6.00  2.00  8.00 CTGGAAAGCACCTGCTCTGT
POSITION PENALTY, QUALITY: 140.000000, 140.588967
RIGHT PRIMER       382   20   59.04   50.00  4.00  0.00 10.00 ATGCAGACAGAAACGCACTC
POSITION PENALTY, QUALITY: 0.000000, 0.959447
SEQUENCE SIZE: 661
INCLUDED REGION SIZE: 661

PRODUCT SIZE: 112, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,65

    0 TTGCTGCCTTCAGGCCTGGGTGGATCAAGCAGCTGTTCACAAATTTCATCTCCTTCACCC
                                                                  

   60 TGAAGCTGGTCCTGAAGGGACAGGTGAGTGAGGCTGGCTGACTCCCTGTGGTCCAGGCCA
                                                                  

  120 TGCCCAGGAGGCTGGATCCCTTTCCTCCCTGCCTTTCCCTGAGAAGGTGCCACTCCCACC
                                                                  

  180 TTCTCCATGTGGCCAGTCCCCTGTGCCGGTCCCCAGCACTGCCACCACCACGCAGCTGGA
                                                                  

  240 AGGAGGCACTCCGTCTGGCCTCCTTTCCTGCCTGGAAAGCACCTGCTCTGTCTGCCCCAG
                                     >>>>>>>>>>>>>>>>>>>>       **

  300 ATCTGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCT
      ************************************************************

  360 GGTGAGTGCGTTTCTGTCTGCATGCCTCAGAAGACAGCAGTGGGAGCCAGAAAGCCACCT
      ***<<<<<<<<<<<<<<<<<<<<                                     

  420 GCTGCACTATGTGGCCTTGGGACTGTCACTCTTCCTGTCTAGGTCCCATGGGCTCTATCT
                                                                  

  480 GGCTCTGACACTTGATGATTAGTTATGAGCATACTTTGGCAAAGCTCTGCCCCTTTGGTG
                                                                  

  540 CGGCTCACAAGCTGTGTGGCGAAGGGCTTGTCTATAGAACTCAGGACAAATGGGTGATTA
                                                                  

  600 AGTCCAAGAGGCATCCAAGATTCTCCTGGAAGTAGATTAGGAAAAAAGATAATTAGATTG
                                                                  

  660 C
       

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3704     0   289     0     0     0     0   262  2572     0     3     0     0     0   578
Right   3593     0   289     0     0    15     0   752  1654     0     0     0    22     0   861
Pair Stats:
considered 102, high any compl 81, high end compl 20, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X8

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   59.81   50.00  4.00  2.00 10.00 CGTTGATCTTTTCCTCCTGC
POSITION PENALTY, QUALITY: 0.000000, 0.186600
RIGHT PRIMER       412   19   62.33   63.16  1.00  0.00 10.00 CCACCCTCCCACAACTCCT
POSITION PENALTY, QUALITY: 0.000000, 3.327467
SEQUENCE SIZE: 692
INCLUDED REGION SIZE: 692

PRODUCT SIZE: 135, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,96

    0 GCTGGGCACTCATCCATGTACTGTACTCTCCTATGCAGTACAGAGCAGAGCTGGGTTTCA
                                                                  

   60 GCCCAAGTCTTGGACTCTGCTCTGAACCAACCTTCTAGAAGGGCTCTACCTACCCAGACA
                                                                  

  120 GACAGACTTGGGAAAAGAGAGAATGAAAAAGTGCCACACCCCTCCCCGCACACCCAGGTC
                                                                  

  180 CCACTTTACAGAGGGGAACACTGAGGCTGGAGGGTTGGGTAGCTGTGTGGATGCAGGGGA
                                                                  

  240 CGGTGACTCAGGGCAATTCCCCCATCCCTGAGGCCCTGCGTTGATCTTTTCCTCCTGCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 CCAGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCA
      ************************************************************

  360 TCACAGCCTCCTACCTGGAGTCCCATCACAAGGTAGGAGTTGTGGGAGGGTGGGCAGGGC
      **********************************<<<<<<<<<<<<<<<<<<<       

  420 CCAGCTTCCCCAGGGGAGTTGGTCCTTTTTTGTGCTCTGACAACCCCATCCCCCAGCTTC
                                                                  

  480 AACCTTATGGCAGCCAAGAGTCCTGGGGAGCTCCTCCTCATTCCTGATGCTCCTCCGCAT
                                                                  

  540 TCCTGATGCTGCGAGGAGGGCAGGCCACAGCGACGTGCCCCTGACCCCTCTCTGCAGGCA
                                                                  

  600 CCAGGGCTGCCCACTACAAGGATCCCAGCAAAGCACCAGCTCCTTCCTAGAGGGCTTATT
                                                                  

  660 CGGCTTCTGTCATCCTCTACAGCAGTGGATTG
                                      

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4014     0   289     0     0     0     0   645  2223     0     0     0     0     0   857
Right   3861     0   289     0     0     8     0   269  2629     0     0     0    22     0   644
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X9

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        145   18   62.86   66.67  3.00  0.00  8.00 AGCGTCCTGGCTTCCTCC
POSITION PENALTY, QUALITY: 0.000000, 4.864632
RIGHT PRIMER       375   18   62.63   66.67  4.00  2.00  8.00 ATCCCCTAGCAGCCCAGC
POSITION PENALTY, QUALITY: 220.000000, 224.633226
SEQUENCE SIZE: 628
INCLUDED REGION SIZE: 628

PRODUCT SIZE: 231, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 163,184

    0 TTTGTTGAATGAGTGAAAGCCGCTGGGGAAACTGGGTACAGCTCTTTCCTCAGTTTCCCC
                                                                  

   60 ATCTGCACTCTGGGCTGAATGCTGGGCTCCTCCCAATCTCCCTGAAGCTGGACCTGAGCC
                                                                  

  120 CAGTAGGGACACACAGGGTCCAGCCAGCGTCCTGGCTTCCTCCAGGGTCATTTCATCTAC
                               >>>>>>>>>>>>>>>>>>*****************

  180 AAGAATGTCTCAGAGGACCTCCCCCTCCCCACCTTCTCGCCCACACTGCTGGGGGACTCC
      ************************************************************

  240 CGCATGCTGTACTTCTGGTTCTCTGAGCGAGTCTTCCACTCGCTGGCCAAGGTAGCTTTC
      ************************************************************

  300 CAGGATGGCCGCCTCATGCTCAGCCTGATGGGAGACGAGTTCAAGGTGAGTGGGTGGGGC
      ***********************************************           <<

  360 TGGGCTGCTAGGGGATCCAGATGGCATGTGGTATGTGTGTGTGTGCACACGCATGGGGAG
      <<<<<<<<<<<<<<<<                                            

  420 GAGGGAGGAAACTCGGAAACTTGGTGGTGGGCAAAAGAACTAAGCTGGAGCAATAGCAGT
                                                                  

  480 GAAGTCCAGACTGGGCACAGTGGCTCACACCTGTAATCCCAATCCTTTGGGAGGCTGAGA
                                                                  

  540 TGTAGCAGGACGAACCGCAGACAAAACTCCTCAGACACTGAGTTAAAGAAGGAAAGAGTT
                                                                  

  600 TATTCAGCCGGGAGCATGGGTAAGACTC
                                  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3527     0   272     0     0     0     0   323  2355     0     0     0     0     0   577
Right   4589     0   154     0     0     0     0   711  2838     0     0     0     0     0   886
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X10

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        213   22   57.47   36.36  3.00  0.00 10.00 TCCAACTTCCTCATTTCTTTTC
POSITION PENALTY, QUALITY: 0.000000, 4.533349
RIGHT PRIMER       319   19   61.92   57.89  4.00  2.00  9.00 ATAAACCCCACACAGGGGC
POSITION PENALTY, QUALITY: 220.000000, 222.919106
SEQUENCE SIZE: 399
INCLUDED REGION SIZE: 399

PRODUCT SIZE: 107, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 235,55

    0 CTTGTCGAGGTCACACAGCATGTGTGGGGCACAGTGGGGAGCAGAAGCCAGCCTCCAGCC
                                                                  

   60 GGACACCCCTTCCCCATTCCACACCCTGCCCAGAGCATCTCACATGTTGTCTGGGAGGTT
                                                                  

  120 GGGAGTTGCGTCTGAGGAGGGGTCCAGTCCTTGAAACTGCCCTTGGTCCCTGCGAAGTTT
                                                                  

  180 TCTTCTGAGGAGTGGACTTTACTCCACCCACCCTCCAACTTCCTCATTTCTTTTCAGGCA
                                       >>>>>>>>>>>>>>>>>>>>>>*****

  240 GTGCTGGAGACCTGGGGCTTCAACACCAACCAGGAAATCTTCCAAGAGGTAACTCCCCCC
      **************************************************          

  300 TGCCCCTGTGTGGGGTTTATCTCACGTACCCCAATCCTGCTCTGGCTTCAAGAGCCCCCA
       <<<<<<<<<<<<<<<<<<<                                        

  360 CACAGCCAATAACACCACCAATGGCAACAATTATAACAG
                                             

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    2785     0   100     0     0     0     0   259  1906     0     0     0     0     0   520
Right   1498     0   226     0     0     0     0   153   792     0     0     0    22     0   305
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X11

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        107   18   60.31   66.67  5.00  0.00  9.00 GGGACCCCTGTCTTCCAC
POSITION PENALTY, QUALITY: 0.000000, 2.311131
RIGHT PRIMER       317   18   61.12   66.67  4.00  0.00 10.00 ACCACCTCTCCCTGCACC
POSITION PENALTY, QUALITY: 120.000000, 123.122481
SEQUENCE SIZE: 409
INCLUDED REGION SIZE: 409

PRODUCT SIZE: 211, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 125,169

    0 CCCCGAGCTACTTCCTTTTCCCAGCCCTGGGGCCCGGAGCCAGCTTTGTGGTTCCCATCT
                                                                  

   60 CCGAGGGCATGGACTGCTGCTCCGTGATGGGCCCCTGTCCTGGCCATGGGACCCCTGTCT
                                                     >>>>>>>>>>>>>

  120 TCCACAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTCCACTGCCTCAAGAT
      >>>>>*******************************************************

  180 GCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCAGTGATGGTGAAATT
      ************************************************************

  240 CCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTTGAAGAGGTGAGGCG
      ******************************************************      

  300 GGTGCAGGGAGAGGTGGTGGTGGGGGAACTGACTCACATATGGGCCGCAGAGGGCAGGGG
      <<<<<<<<<<<<<<<<<<                                          

  360 CTGGGGGTCTCTGAAGCCTCCAGATCCTTCTCACCACCTCTGCTGGCAC
                                                       

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    2831     0   106     0     0    50     0   286  1982     0     0     0     0     0   407
Right   2741     0   116     0     0    42     0   336  1775     0     0     0     0     0   472
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X12

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   58.48   45.00  8.00  0.00 11.00 CAAATTTGGTTTCTCTCCCC
POSITION PENALTY, QUALITY: 0.000000, 1.522783
RIGHT PRIMER       391   22   61.09   50.00  6.00  3.00 10.00 CCTTCTCTTCTCTGCAGCACAT
POSITION PENALTY, QUALITY: 0.000000, 3.085335
SEQUENCE SIZE: 668
INCLUDED REGION SIZE: 668

PRODUCT SIZE: 114, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,72

    0 GTTGCAGTGAGCTGAGATCGTGCCACTGCCCTCCAGCCTGGGCAACAGAGTGAGACTGTC
                                                                  

   60 TCAAAAACAAAAAAAGAAAAGAAAAGAAAAAGAAAGTGACTTCTCAGGTCCTAACCCCAA
                                                                  

  120 AGCCACAGGTGCTGGGGAACTTTCCTCGGTTTTCAGAAGAGCAGTAGCTAAGCCTGGTTC
                                                                  

  180 CCGTGTCATCCTTGCCTCTCCAGTCCCTCAGTGGAAAGAATCAGGGGCCCTGAGCTAGGA
                                                                  

  240 GGGTTGCTCTCTGCTTCGGGAAGAGCCCTGGCTCACAGCAAATTTGGTTTCTCTCCCCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 GATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGCCTCTTG
      ************************************************************

  360 GATTTCCAGTATGTGCTGCAGAGAAGAGAAGGGGGCGGTCAACTCCGCAAACCTCTCCCT
      **********<<<<<<<<<<<<<<<<<<<<<<                            

  420 GGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGGTGGGGAAATGTGGCCCCTTTCTTCT
                                                                  

  480 GGGGCATATGGGCTGACTGCAGGGAAGATAAGACCCTGCCTAGATAGAATCTTCGTGGGG
                                                                  

  540 AAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAGGAAGAAGGCCTGAGCTATGAGACAA
                                                                  

  600 AAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCAGGGGATGTTACAGGAGGGGGCCCAA
                                                                  

  660 TGGAGGGT
              

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3686     0   289     0     0   114     0   669  1809     0     0     0    14     0   791
Right   3774     0   289     0     0     0     0   619  2094     0     0     0     0     0   772
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X13

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        269   23   60.53   34.78  4.00  0.00  9.00 GGGTCAAATTATCATCGCTTTTT
POSITION PENALTY, QUALITY: 120.000000, 123.526785
RIGHT PRIMER       358   23   59.90   52.17  6.00  0.00  8.00 CCCCAGTCTATCCAAGACTACCT
POSITION PENALTY, QUALITY: 0.000000, 3.097964
SEQUENCE SIZE: 634
INCLUDED REGION SIZE: 634

PRODUCT SIZE: 90, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,38

    0 CGGTCAACTCCGCAAACCTCTCCCTGGCCCCTTGGAGTCAGGCACAGGGCGGGGTGTTGG
                                                                  

   60 TGGGGAAATGTGGCCCCTTTCTTCTGGGGCATATGGGCTGACTGCAGGGAAGATAAGACC
                                                                  

  120 CTGCCTAGATAGAATCTTCGTGGGGAAGAAGGGGCTCCAGGAAGAATGGAGGGCTGCCAG
                                                                  

  180 GAAGAAGGCCTGAGCTATGAGACAAAAGCACTGGCTGCTATTCTTAGAGTTTCTTTCCCA
                                                                  

  240 GGGGATGTTACAGGAGGGGGCCCAATGGAGGGTCAAATTATCATCGCTTTTTTATTTCAG
                                   >>>>>>>>>>>>>>>>>>>>>>>      **

  300 GATTACACCAAAGACTGTTTCCAACTTGACTGAGGTAGGTAGTCTTGGATAGACTGGGGG
      ************************************<<<<<<<<<<<<<<<<<<<<<<< 

  360 AAATAAGTCCTGTGGGACCTCCTGCCTTAAAGAAAGCAGGCGGAGGGCCCTAAAGGAAAT
                                                                  

  420 CAGGCAACCAGACCAAAAGAATGTGACCAGGTGGTCCATGCTGTGTCTCTTGTGACCCTT
                                                                  

  480 CTTCTCCCTGCCATGTCTTTTGGGAGAGCCCTTGTGTTGCAAAAATGAGAGTGTGGTGGT
                                                                  

  540 ATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAAGCAGCGCTCCCTGGACCTCAATTTT
                                                                  

  600 CCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTC
                                        

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3282     0   289     0     0     1     0   523  1815     0     0     0    22     0   632
Right   3434     0   289     0     0     0     0   586  1682     0     0     0     0     0   877
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X14

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   19   60.74   47.37  6.00  2.00  9.00 CACCATGGGCATTTGATTG
POSITION PENALTY, QUALITY: 20.000000, 21.740693
RIGHT PRIMER       396   18   62.83   66.67  2.00  0.00  8.00 GTTTCCCCTCCAGCCCAC
POSITION PENALTY, QUALITY: 80.000000, 84.832325
SEQUENCE SIZE: 436
INCLUDED REGION SIZE: 436

PRODUCT SIZE: 119, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,77

    0 GTTGCAAAAATGAGAGTGTGGTGGTATGGATTGGGGTTTAGGCAGAACAGTACTGGCCAA
                                                                  

   60 GCAGCGCTCCCTGGACCTCAATTTTCCCTCTGTGGAATGGGCTAGCAATCCTGGGCCTCC
                                                                  

  120 CCAGGGCGAAGGAAAGACCACTCAGGAAGGGCACCGTCTGGGGCAGGAAAACGGAGTGGG
                                                                  

  180 TTGGATGTATTTTTTTCACGGATGGGCATGAGGATGAATGCTTGTCCAGGCCGTGCAGCA
                                                                  

  240 TCTGCCTTGTGGGTCACTTCTGTGCTCCAGGGAGGACTCACCATGGGCATTTGATTGCAG
                                            >>>>>>>>>>>>>>>>>>> **

  300 AGCAGCTCCGAGTCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCT
      ************************************************************

  360 GAGGTCATGTCTCGTAAGTGTGGGCTGGAGGGGAAACTGGGTGCCGAGGCTGACAGAGCT
      ***************    <<<<<<<<<<<<<<<<<<                       

  420 TCCCATTTCACCTTTT
                      

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3425     0    52     0     0     3     0   235  2489     0     2     0    23     0   621
Right   1454     0   289     0     0     0     0    83   882     0     0     0     0     0   200
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X15

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        231   18   62.06   66.67  4.00  1.00  9.00 ACTGGGGCTCTGTCCCCT
POSITION PENALTY, QUALITY: 100.000000, 104.061036
RIGHT PRIMER       377   18   61.67   61.11  3.00  1.00 11.00 AGGAACAGGGGCTGGTGA
POSITION PENALTY, QUALITY: 320.000000, 323.670770
SEQUENCE SIZE: 642
INCLUDED REGION SIZE: 642

PRODUCT SIZE: 147, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 254,90

    0 GGATGGGTTGGGAGCTCAAGTTTTGGGGCAGAAGGGAATTTTTTTTGGCAGCAGAGTGCA
                                                                  

   60 AGCCCTGCCGCCAGGCAAACTCTGCTCTTCCTCATCCTCAGAAGCACTTGCTCACTCTGC
                                                                  

  120 TAAATCAAAGTGAAACGCATGTTTACAGAATATTGGTCCAAAAGGGTCTCAGCATCTCCC
                                                                  

  180 ACTACCCAGGGTGCAGAGCCTCGGGCCGGCCTTGCTCCCCAAGAAGGGCTGACTGGGGCT
                                                         >>>>>>>>>

  240 CTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTG
      >>>>>>>>>     **********************************************

  300 AGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGATGTGAGTACAAAGCCCCCC
      ********************************************                

  360 TCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATTCCTGGGGTGACTGGGGGC
      <<<<<<<<<<<<<<<<<<                                          

  420 TGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCCCTCCTGGTGGCCTGGGAG
                                                                  

  480 TCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAGGGCTTCCTGCTGCTGCAG
                                                                  

  540 ATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAGAAG
                                                                  

  600 TCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC
                                                

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3343     0   289     0     0    24     0   488  1840     0     0     0    24     0   678
Right   3758     0   245     0     0     1     0   356  2464     0     0     3    22     0   667
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR CETP_X16

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   62.38   65.00  2.00  0.00  9.00 CTCCTACTGCCCCTCCCTTC
POSITION PENALTY, QUALITY: 0.000000, 2.381034
RIGHT PRIMER       394   18   60.61   66.67  6.00  2.00  8.00 CCCGACCTCCTTGGAGAC
POSITION PENALTY, QUALITY: 0.000000, 2.609852
SEQUENCE SIZE: 672
INCLUDED REGION SIZE: 672

PRODUCT SIZE: 117, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,79

    0 GAAGGGCTGACTGGGGCTCTGTCCCCTCGCCCAGGGCTCGAGGTAGTGTTTACAGCCCTC
                                                                  

   60 ATGAACAGCAAAGGCGTGAGCCTCTTCGACATCATCAACCCTGAGATTATCACTCGAGAT
                                                                  

  120 GTGAGTACAAAGCCCCCCTCACCAGCCCCTGTTCCTGGGGAGAGAGGCCCAGACAGGATT
                                                                  

  180 CCTGGGGTGACTGGGGGCTGTTGGGGAGACAGACAGAGGGGCCTCTACCAGCTTGGCTCC
                                                                  

  240 CTCCTGGTGGCCTGGGAGTCAGCCCAGCTCGCCCCTCTCTCCTACTGCCCCTCCCTTCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 GGCTTCCTGCTGCTGCAGATGGACTTTGGCTTCCCTGAGCACCTGCTGGTGGATTTCCTC
      ************************************************************

  360 CAGAGCTTGAGCTAGAAGTCTCCAAGGAGGTCGGGATGGGGCTTGTAGCAGAAGGCAAGC
      *****************<<<<<<<<<<<<<<<<<<                         

  420 ACCAGGCTCACAGCTGGAACCCTGGTGTCTCCTCCAGCGTGGTGGAAGTTGGGTTAGGAG
                                                                  

  480 TACGGAGATGGAGATTGGCTCCCAACTCCTCCCTATCCTAAAGGCCCACTGGCATTAAAG
                                                                  

  540 TGCTGTATCCAAGAGCTGCGGAGTCCTTCTTCTGTGGCTGGCGGGTAGAGGGGGGGGGAA
                                                                  

  600 GGGATTGTCTCACCAGTGCCGTCCACCTCTTTTCAGCCCTTCCAAGCAGCTGCCCCCAAA
                                                                  

  660 CCCTCCAAGCTT
                  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3689     0   286     0     0     3     0   288  2492     0     0     0    22     0   598
Right   3637     0   289     0     0     3     0   299  2316     0     0     0    25     0   705
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X1

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        280   18   59.14   66.67  4.00  1.00 11.00 CCAGAGGGGTCAGGACAG
POSITION PENALTY, QUALITY: 0.000000, 2.861995
RIGHT PRIMER       430   18   61.13   61.11  6.00  1.00 11.00 AGCCTGCAAGCACTCCCT
POSITION PENALTY, QUALITY: 20.000000, 23.125223
SEQUENCE SIZE: 710
INCLUDED REGION SIZE: 710

PRODUCT SIZE: 151, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,114

    0 AAGCAACCACCGCTATATGTTAGGATTCGAAGGAGCTCCAGGAAAGTCTCATAGCCCCAC
                                                                  

   60 TGGCCAGAATGGGCTAAATCTCAGAGGGGGAGGGTGGGAGATGGGGGTGACAGTGACCTT
                                                                  

  120 TTTTGTGACTCCTCCTAGACCATCCATCCCTGCTCCCAGGAGGACCTGTCCTCCCAGATG
                                                                  

  180 GTGGAGATGGACAGGAGGACTATCTACCCACCCGTCCCCACGGCCCTGACCCTCTGACCT
                                                                  

  240 CACCCTCTCCGCTGATTTCTTCATGTTAGTTCAACATTACCCAGAGGGGTCAGGACAGAC
                                              >>>>>>>>>>>>>>>>>>**

  300 AATTCCTCAGTGACCCAGGAGCTGACACACTATGGCGCACGTCCGAGGCTTGCAGCTGCC
      ************************************************************

  360 TGGCTGCCTGGCCCTGGCTGCCCTGTGTAGCCTTGTGCACAGCCAGCATGGTAAGGGAGT
      **************************************************** <<<<<<<

  420 GCTTGCAGGCTGGAACAGGCTGGAGGACTGGGGTGTGGGCCCATGGGCTGGGGTCTCCTG
      <<<<<<<<<<<                                                 

  480 GCTGGACAGAGCACACAGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTA
                                                                  

  540 GGGAAGAAGTCAGGAGCTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAG
                                                                  

  600 GGAGCAGGCTGGGGGCAAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCC
                                                                  

  660 TTCCAGGCCTTCCACCAGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGG
                                                        

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4041     0   289     0     0    21     0   498  2476     0     0     0    22     0   735
Right   4194     0   289     0     0     8     0   235  3082     0     0     2     0     0   578
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X2

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        265   18   60.05   61.11  4.00  0.00  9.00 GTCGCTGTTCCATGACCC
POSITION PENALTY, QUALITY: 300.000000, 302.054062
RIGHT PRIMER       516   19   61.43   63.16  6.00  2.00  7.00 GTCTAGAGTTGGGCCCGGT
POSITION PENALTY, QUALITY: 700.000000, 702.432556
SEQUENCE SIZE: 761
INCLUDED REGION SIZE: 761

PRODUCT SIZE: 252, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,165

    0 AGAGCTGGCCCCTAAGTAGGTCTCAGCCCCAGGCGGCCAGCTTAGGGAAGAAGTCAGGAG
                                                                  

   60 CTCAGGGCTGGAAAGAGAATGGCTGCTTCTCTCTTCCAATATAGGGAGCAGGCTGGGGGC
                                                                  

  120 AAGGGGCAGTGTAGGAGGGGCACAGGGGGCCACATTTAGCAGCCTTCCAGGCCTTCCACC
                                                                  

  180 AGCCCAGACTGCCTCTCTCAGAAGCCAGCAGGGGAGGGTGGGCTTGCTTCATGCCCCCAG
                                                                  

  240 ATGGCCAAGACTGCCTGTTCCTGAGGTCGCTGTTCCATGACCCCCCCACCGCCTTTACAG
                               >>>>>>>>>>>>>>>>>>               **

  300 TGTTCCTGGCTCCTCAGCAAGCACGGTCGCTGCTCCAGCGGGTCCGGCGAGCCAACACCT
      ************************************************************

  360 TCTTGGAGGAGGTGCGCAAGGGCAACCTAGAGCGAGAGTGCGTGGAGGAGACGTGCAGCT
      ************************************************************

  420 ACGAGGAGGCCTTCGAGGCTCTGGAGTCCTCCACGGCTACGGTGAGCCTGGGCTGCTCGG
      *******************************************                 

  480 ACGGTGCCGGGGCCTCAGACCGGGCCCAACTCTAGACACTTCCACAGAGAAGCAAGCGAG
                        <<<<<<<<<<<<<<<<<<<                       

  540 GAACGCCACAGCCCCTTCGCTGCTCACAGCCTCATTTCAACTCTGAGCCCCTCCTCACAG
                                                                  

  600 GGCTGGCAAGAGGAGCGGCCTCAGCCTTTCCTGGGGGTCTCTGTGCCTGGACTGTGTCCC
                                                                  

  660 TGTGCAGCTCCATGACATGGGGAGGCCTCCACAGTCTTCAGACATCCACCTGCCTTGGAG
                                                                  

  720 CTCTGTGTCCACATGGCCTCCTCAGCGGCAGACTCCCACAC
                                               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4542     0   289     0     0    14     0   245  3332     0     0     0    23     0   639
Right   4704     0   289     0     0    96     0   191  3583     0     4     0     0     0   541
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X3

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   60.48   50.00  2.00  0.00 11.00 ACTCCCAGCCCTTGTTTTTC
POSITION PENALTY, QUALITY: 0.000000, 0.475706
RIGHT PRIMER       344   18   60.01   55.56  4.00  2.00  8.00 AAATCCTTCCCGGTGCTC
POSITION PENALTY, QUALITY: 0.000000, 2.010701
SEQUENCE SIZE: 625
INCLUDED REGION SIZE: 625

PRODUCT SIZE: 67, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,29

    0 TGTTCCTGGAGCTCTGTGTCGCCTTTCCTGTCTGTAGGGACTCTGCCAGGGACCCACTGC
                                                                  

   60 CCCCTCTCCTCCCATCTCCCCCAGCCTCTTTCAGACTCGGTGTGTGTGTTGGAGGAACTC
                                                                  

  120 CCCTATCCTCAAATATTCTTCTCCTTTTGGAAACAAAAGTAGGAAACTCTGCCACAAACC
                                                                  

  180 TCCCCAGAGCCTGCCCCCTGCGTGACCAGGGTAAGGAAAGTGTGAGGAGGAGCATAACAT
                                                                  

  240 TTACTAAAACAACACAAAACAGGAGCTGCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 GATGTGTTCTGGGCCAAGTACACAGGTGAGCACCGGGAAGGATTTGCCCCAGGAAGGGAG
      ***************************<<<<<<<<<<<<<<<<<<               

  360 GCCTGGGGACCCCAGTGAGAGAATTCTACCCAGAGAATCTTCTGCTGCACCTAGCCATCC
                                                                  

  420 ACCCATCCACCCCTTCCCCACTCCTTCCTTGGTCCCTCCCATCTGTTCATCCATCTTTCT
                                                                  

  480 GTTTCTCACCAACATCCCATCCACCCTGACTCCAGCTCATCCTGGCCATACCCCAATCCC
                                                                  

  540 AAAGGTAAACACCTGGGTCTTTTCCAGCTTGTGAGACAGCGAGGACGCCTCGAGATAAGC
                                                                  

  600 TTGCTGCATGTCTGGAAGGTGAGCA
                               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3344     0   289     0     0     7     0   659  1679     0     0     0     0     0   710
Right   3344     0   289     0     0     1     0   379  2040     0     0     0     0     0   635
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X4

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        271   21   60.63   52.38  4.00  2.00  8.00 CCAAAGGTAAACACCTGGGTC
POSITION PENALTY, QUALITY: 120.000000, 121.626330
RIGHT PRIMER       372   20   61.69   55.00  4.00  0.00  8.00 CAAACCCGTGTCAGTTGCTC
POSITION PENALTY, QUALITY: 0.000000, 1.693157
SEQUENCE SIZE: 651
INCLUDED REGION SIZE: 651

PRODUCT SIZE: 102, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,55

    0 GCCGTAGCCTCACTCCCAGCCCTTGTTTTTCAGGATGTGTTCTGGGCCAAGTACACAGGT
                                                                  

   60 GAGCACCGGGAAGGATTTGCCCCAGGAAGGGAGGCCTGGGGACCCCAGTGAGAGAATTCT
                                                                  

  120 ACCCAGAGAATCTTCTGCTGCACCTAGCCATCCACCCATCCACCCCTTCCCCACTCCTTC
                                                                  

  180 CTTGGTCCCTCCCATCTGTTCATCCATCTTTCTGTTTCTCACCAACATCCCATCCACCCT
                                                                  

  240 GACTCCAGCTCATCCTGGCCATACCCCAATCCCAAAGGTAAACACCTGGGTCTTTTCCAG
                                     >>>>>>>>>>>>>>>>>>>>>      **

  300 CTTGTGAGACAGCGAGGACGCCTCGAGATAAGCTTGCTGCATGTCTGGAAGGTGAGCAAC
      *****************************************************<<<<<<<

  360 TGACACGGGTTTGGGGAGCAGGACATGGAGGGGAGCTTGGGAGAAGAGCTCAGGGGTGGG
      <<<<<<<<<<<<<                                               

  420 TTTGGAGTGTGGCTGGTGGAGGCCGAGGCAGTCCCCAGCATCTGACATTGCTCCCATTCC
                                                                  

  480 TGGGGTCAAGATGTCTCTTTGTACCTGGCTCTGTGTCTGGCATGCGAACGAATGAATGAA
                                                                  

  540 TGAATGGACTAATGAATTAATGTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTGCCCAG
                                                                  

  600 GCTGGAGTGCAGTGGCACGATCTTGGCTCACTGTAAACTCCGCCTCCCGGA
                                                         

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3604     0   289     0     0     1     0   409  2209     3    14     0     0     0   679
Right   3497     0   289     0     0   107     0   371  2132     0     0     0    18     0   580
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X5

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        264   18   61.97   66.67  5.00  1.00  9.00 CAGGCTCCAAGGCTGACC
POSITION PENALTY, QUALITY: 320.000000, 323.965248
RIGHT PRIMER       425   18   62.59   66.67  4.00  0.00  8.00 AAGGCCGACCCCTCACTC
POSITION PENALTY, QUALITY: 0.000000, 4.589237
SEQUENCE SIZE: 706
INCLUDED REGION SIZE: 706

PRODUCT SIZE: 162, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,110

    0 TGCTGGCCAAGAGAAGCCACAGAATGGAAGCTCCATGAGGGCAGGGCTGTGACTGTCCTA
                                                                  

   60 TTGGTTGATGTGTACTGAGCACCCGACAGTGCCTGTCATATGGTAGGCACTTAGCGAATA
                                                                  

  120 TTTGGAGGCCACTGTTGAGTGAATGGGAGAACTGCTGGTTGCAGAGGAAGAGGGGCTGGG
                                                                  

  180 TGAATGCAGGTTCAGGATTGTGGACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTT
                                                                  

  240 GCAGGGAGAGAGGAAATAAGTCCCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAG
                              >>>>>>>>>>>>>>>>>>                **

  300 GTAACTGTGCTGAGGGTCTGGGTACGAACTACCGAGGGCATGTGAACATCACCCGGTCAG
      ************************************************************

  360 GCATTGAGTGCCAGCTATGGAGGAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTC
      ************************************************<<<<<<<<<<<<

  420 GGCCTTCCCACCATGGGCTGAGAACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCT
      <<<<<<                                                      

  480 GTTGGGCAATTTCCTCTTCCAGAATCAACTCCACTACCCATCCTGGGGCCGACCTACAGG
                                                                  

  540 AGAATTTCTGCCGCAACCCCGACAGCAGCACCACGGGACCCTGGTGCTACACTACAGACC
                                                                  

  600 CCACCGTGAGGAGGCAGGAATGCAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGC
                                                                  

  660 GACCCATGACCAAGCCCGGGGGCTTCATGGGGCCTGGCAGCCTGGG
                                                    

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4154     0   289     0     0     2     0   468  2494     0     0     0     0     0   901
Right   4154     0   289     0     0    14     0   310  2916     0     0     0     0     0   625
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X6

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   60.81   50.00  6.00  0.00 11.00 GTTGGGCAATTTCCTCTTCC
POSITION PENALTY, QUALITY: 0.000000, 0.806671
RIGHT PRIMER       515   18   61.28   61.11  3.00  3.00  9.00 CTTGGTTCCCATCCCAGG
POSITION PENALTY, QUALITY: 1180.000000, 1183.280619
SEQUENCE SIZE: 737
INCLUDED REGION SIZE: 737

PRODUCT SIZE: 238, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,141

    0 GACCTGCATGAGCTGGGAGGTGGGGGATAGACAACTTTGCAGGGAGAGAGGAAATAAGTC
                                                                  

   60 CCCAGGCTCCAAGGCTGACCGGGGTGGGGTCTCCGCAGGTAACTGTGCTGAGGGTCTGGG
                                                                  

  120 TACGAACTACCGAGGGCATGTGAACATCACCCGGTCAGGCATTGAGTGCCAGCTATGGAG
                                                                  

  180 GAGTCGCTACCCACATAAGCCTGAGTGAGTGAGGGGTCGGCCTTCCCACCATGGGCTGAG
                                                                  

  240 AACAGGGAGCAAGCGTACCTCAAGTTCAACAGCCTCCTGTTGGGCAATTTCCTCTTCCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 AATCAACTCCACTACCCATCCTGGGGCCGACCTACAGGAGAATTTCTGCCGCAACCCCGA
      ************************************************************

  360 CAGCAGCACCACGGGACCCTGGTGCTACACTACAGACCCCACCGTGAGGAGGCAGGAATG
      ************************************************************

  420 CAGCATCCCTGTCTGTGGTAGGCTGGGGGCAGTGGGGCGACCCATGACCAAGCCCGGGGG
      *******************                                         

  480 CTTCATGGGGCCTGGCAGCCTGGGATGGGAACCAAGAATACTGGCTACCCAGGCACAGTG
                        <<<<<<<<<<<<<<<<<<                        

  540 GCTCATGCCCGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACCTGAGGTCAG
                                                                  

  600 GGGTTTGAGACCAGCTGGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAAT
                                                                  

  660 TGCCAGGCGTGGTGGTGGGCGCCTGTAATCCCAACTACTCTGGAGGCTGAGGCACGAGAA
                                                                  

  720 TCGCTTGAACCCGGGAG
                       

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4464     0   289     0     0     2     0   392  2999     0     0     0     0     0   782
Right   4464     0   289     0     0    40     0   325  3245     0     0     0     0     0   565
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X7

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        269   18   62.25   66.67  3.00  1.00 11.00 CTCTGCTGCCTCCTTGCC
POSITION PENALTY, QUALITY: 220.000000, 224.254433
RIGHT PRIMER       641   18   62.95   72.22  4.00  1.00  8.00 CTCAGGCCCCCTACCCAG
POSITION PENALTY, QUALITY: 140.000000, 144.945754
SEQUENCE SIZE: 915
INCLUDED REGION SIZE: 915

PRODUCT SIZE: 373, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,319

    0 GCAGCTCCAGGCAGCCACTACCAGTTGGTTACAGATGGCCTAGGAGGCCAAACCTGGTTA
                                                                  

   60 CTATCTCTGGTTTATTATGTGCCAGACACTTATGCTGTATATTTTGTTTAATCCTCTCAA
                                                                  

  120 CAAACCTGCAAAAGTGGCATTAGTAACCCCTTTAAAGGCAAACGGTCAGAAGCCCAGAGA
                                                                  

  180 GGTTAAGTAACCTGAGGTCACACAGGCAGAAAGCAGCAAGACCGGGGTTCACACCCCTGT
                                                                  

  240 CTGTTCCGGTCCATGTGTGGTCTCACTCACTCTGCTGCCTCCTTGCCCCTCACCCACCAG
                                   >>>>>>>>>>>>>>>>>>           **

  300 GCCAGGATCAAGTCACTGTAGCGATGACTCCACGCTCCGAAGGCTCCAGTGTGAATCTGT
      ************************************************************

  360 CACCTCCATTGGAGCAGTGTGTCCCTGATCGGGGGCAGCAGTACCAGGGGCGCCTGGCGG
      ************************************************************

  420 TGACCACACATGGGCTCCCCTGCCTGGCCTGGGCCAGCGCACAGGCCAAGGCCCTGAGCA
      ************************************************************

  480 AGCACCAGGACTTCAACTCAGCTGTGCAGCTGGTGGAGAACTTCTGCCGCAACCCAGACG
      ************************************************************

  540 GGGATGAGGAGGGCGTGTGGTGCTATGTGGCCGGGAAGCCTGGCGACTTTGGGTACTGCG
      ************************************************************

  600 ACCTCAACTATTGTGGTGAGCTGCCTGGGTAGGGGGCCTGAGTTGCAGGGACAAATCCTA
      *****************       <<<<<<<<<<<<<<<<<<                  

  660 GTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTTACCTCATTGAGTGCGCTCATT
                                                                  

  720 ACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTGCCCATTTCACAGATAAGTACA
                                                                  

  780 CTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGTGCATGCACGCTTAACCTCTGC
                                                                  

  840 ACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAAT
                                                                  

  900 GGCCCAGCCCAGTCC
                     

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    6244     0   289     0     0    60     0   973  3795     0     0     0     0     0  1127
Right   5938     0   289     0     0    51     0   580  3983     0     0     0    44     0   991
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X8

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        246   18   61.28   61.11  2.00  2.00  9.00 GGATGGGTGAGGAATGGC
POSITION PENALTY, QUALITY: 680.000000, 683.278099
RIGHT PRIMER       450   19   62.38   63.16  3.00  0.00  8.00 CCTCGGATGCCCACTACCT
POSITION PENALTY, QUALITY: 20.000000, 23.380210
SEQUENCE SIZE: 729
INCLUDED REGION SIZE: 729

PRODUCT SIZE: 205, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,133

    0 GAGTTGCAGGGACAAATCCTAGTGGGAATAACAACAGCCGCTTCTGCTTATCGAACGCTT
                                                                  

   60 ACCTCATTGAGTGCGCTCATTACAGCCTTACAGTAACCAGGTGGGGGGTAAGGTCCTGTG
                                                                  

  120 CCCATTTCACAGATAAGTACACTGAGGCCCCAGGAGGTTATTGCCTAGTAGCCCAACTGT
                                                                  

  180 GCATGCACGCTTAACCTCTGCACCAAATGGCCTCCAAGGCCCGTAGGGGAACTGGGGGGA
                                                                  

  240 TCTAGGGGATGGGTGAGGAATGGCCCAGCCCAGTCCCGGCCGGTGCCTGGGTCCCAACAG
            >>>>>>>>>>>>>>>>>>                                  **

  300 AGGAGGCCGTGGAGGAGGAGACAGGAGATGGGCTGGATGAGGACTCAGACAGGGCCATCG
      ************************************************************

  360 AAGGGCGTACCGCCACCAGTGAGTACCAGACTTTCTTCAATCCGAGGACCTTTGGCTCGG
      ************************************************************

  420 GAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGGATGCGGGGCTGCGGGGCTGGTGGCCAG
      *********** <<<<<<<<<<<<<<<<<<<                             

  480 GACTTGCCCCTCACTGCTTGGCTTGCTCTGCAGACTGTGGGCTGCGACCTCTGTTCGAGA
                                                                  

  540 AGAAGTCGCTGGAGGACAAAACCGAAAGAGAGCTCCTGGAATCCTACATCGACGGGCGCA
                                                                  

  600 TTGTGGAGGGCTCGGATGCAGAGATCGGCATGTCACCTTGGTGTGTCCTGGAGCCCTGCG
                                                                  

  660 CTACCATTCACTCCTGGGGGCAGGTGTGCTGCTGGACCCCCACCCTCAGGCCCTGCCTGC
                                                                  

  720 AGGCCTGGG
               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4078     0   289     0     0    87     0   484  2399     0     1     0    44     0   774
Right   4384     0   289     0     0    97     0   244  3201     0     0     0     0     0   553
Pair Stats:
considered 14, high end compl 12, ok 2
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X9

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        279   19   61.85   57.89  2.00  2.00  9.00 ACTGCTTGGCTTGCTCTGC
POSITION PENALTY, QUALITY: 0.000000, 2.845950
RIGHT PRIMER       454   18   61.32   61.11  5.00  3.00  8.00 AATGGTAGCGCAGGGCTC
POSITION PENALTY, QUALITY: 160.000000, 163.321659
SEQUENCE SIZE: 727
INCLUDED REGION SIZE: 727

PRODUCT SIZE: 176, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,131

    0 CCAAGGCCCGTAGGGGAACTGGGGGGATCTAGGGGATGGGTGAGGAATGGCCCAGCCCAG
                                                                  

   60 TCCCGGCCGGTGCCTGGGTCCCAACAGAGGAGGCCGTGGAGGAGGAGACAGGAGATGGGC
                                                                  

  120 TGGATGAGGACTCAGACAGGGCCATCGAAGGGCGTACCGCCACCAGTGAGTACCAGACTT
                                                                  

  180 TCTTCAATCCGAGGACCTTTGGCTCGGGAGAGGCAGGTGAGGTAGTGGGCATCCGAGGGG
                                                                  

  240 ATGCGGGGCTGCGGGGCTGGTGGCCAGGACTTGCCCCTCACTGCTTGGCTTGCTCTGCAG
                                             >>>>>>>>>>>>>>>>>>>**

  300 ACTGTGGGCTGCGACCTCTGTTCGAGAAGAAGTCGCTGGAGGACAAAACCGAAAGAGAGC
      ************************************************************

  360 TCCTGGAATCCTACATCGACGGGCGCATTGTGGAGGGCTCGGATGCAGAGATCGGCATGT
      ************************************************************

  420 CACCTTGGTGTGTCCTGGAGCCCTGCGCTACCATTCACTCCTGGGGGCAGGTGTGCTGCT
      *********        <<<<<<<<<<<<<<<<<<                         

  480 GGACCCCCACCCTCAGGCCCTGCCTGCAGGCCTGGGCTTTACAGATGACAACAGCTGAGC
                                                                  

  540 ATCCAGGATCCCACCAACTCCACACAGCAGCCACATGAGATGGGTTGTTTACTTCTTTTT
                                                                  

  600 TTTTTGTTTCTTAGATGGAGTCTTGCTCTGTCACCTAGGCTGGAGTGCAGTGCTGCAATC
                                                                  

  660 TCGGCTCACTACCTCGATCTCAGCTCACTGCAACTTCTGCCTTCCGGGTTCAAACGATTC
                                                                  

  720 TCTTGCC
             

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4212     0   289     0     0   182     0   233  2939     0     0     0    22     0   547
Right   4248     0   289     0     0    80     0   415  2693     0     0     0    23     0   748
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X10

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        274   19   60.89   57.89  3.00  0.00  8.00 CTAATGCTTCCTGCTGCCC
POSITION PENALTY, QUALITY: 100.000000, 101.889081
RIGHT PRIMER       487   18   61.58   61.11  4.00  2.00 10.00 ACGGGCCACCAGTTCTGT
POSITION PENALTY, QUALITY: 0.000000, 3.583075
SEQUENCE SIZE: 768
INCLUDED REGION SIZE: 768

PRODUCT SIZE: 214, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,172

    0 CCTGAGCCCAAGAGGCTGAGGCTTCAGTAAGCTGTGACTGTACCATTGCACTCCAGCCTG
                                                                  

   60 GGTGACAAGAGTGAGACCCTGTCTCAAAAATAAATAAATAAATAAATAAAAAGTGTGAGG
                                                                  

  120 CAGCCCCTCAGCATCACACGGAGGCTCCAGCCCCAAAGGCGGCCAGCCCAAGCTTGGATC
                                                                  

  180 TGGGCCCCGGAGGCAGCTCTGCCCAGCTGGGTTCTTAGACCTGGGATTGTTACTTCTAGG
                                                                  

  240 GCTGGTGTAGAGGCAGCCCCCTCATCCTCAGCTCCTAATGCTTCCTGCTGCCCCTCCCAG
                                        >>>>>>>>>>>>>>>>>>>     **

  300 GCAGGTGATGCTTTTCCGGAAGAGTCCCCAGGAGCTGCTGTGTGGGGCCAGCCTCATCAG
      ************************************************************

  360 TGACCGCTGGGTCCTCACCGCCGCCCACTGCCTCCTGTACCCGCCCTGGGACAAGAACTT
      ************************************************************

  420 CACCGAGAATGACCTTCTGGTGCGCATTGGCAAGCACTCCCGCACAAGGTACAGAACTGG
      **************************************************<<<<<<<<<<

  480 TGGCCCGTGGGTGTCTGGCAGGGGTCTGAGTCCTCCAAAGCGATCATGAGGGGCCTTGGT
      <<<<<<<<                                                    

  540 GGCTCCGGGACACATAGGATGTTCTGTATACCCCCCAGAATATAACATCCCAGCAGTCTC
                                                                  

  600 TGCTGGAAAGCCATTTGGTCACGTCCTGACTGAGGCTTGGAGCGCGGGGAGAATCCGTCT
                                                                  

  660 GTCTCTGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCT
                                                                  

  720 GTCTGGACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGC
                                                      

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4774     0   289     0     0   210     0   485  3153     0     0     0     0     0   637
Right   4621     0   289     0     0     8     0   474  3110     0     0     0    22     0   718
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X11

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   59.41   50.00  2.00  2.00  9.00 TTCTTTCTTGGGGTCTCTGC
POSITION PENALTY, QUALITY: 0.000000, 0.594713
RIGHT PRIMER       493   18   62.11   61.11  4.00  3.00  9.00 ACAAGCATCTGGTGGCCC
POSITION PENALTY, QUALITY: 0.000000, 4.108476
SEQUENCE SIZE: 774
INCLUDED REGION SIZE: 774

PRODUCT SIZE: 216, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,178

    0 TGGTCCCTCCAACACTAGGATATAGCCCATGTGGGAGTCTCTGAAAATAGAGTCTGTCTG
                                                                  

   60 GACTAGGGCGTGCAGCCTGTGCCCCTGTCCCCGTCCTCCAGGCTGTCTGACTCCAAAGCC
                                                                  

  120 CTGCACGGCTTTAGGCCCAGGAAGAAACACCCAGGGGGCTGCCATGGCAGGAACCAGCCC
                                                                  

  180 TATCCCCTCCCTGGTGGCCTGCAGGACACACTGTCTCCCAGAACCCCAAGGGCAGGCAGT
                                                                  

  240 TTCCTGCTCCTTGCTGGGTGAACCTGCAGCTTCTCCATTTCTTTCTTGGGGTCTCTGCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 GTACGAGCGAAACATTGAAAAGATATCCATGTTGGAAAAGATCTACATCCACCCCAGGTA
      ************************************************************

  360 CAACTGGCGGGAGAACCTGGACCGGGACATTGCCCTGATGAAGCTGAAGAAGCCTGTTGC
      ************************************************************

  420 CTTCAGTGACTACATTCACCCTGTGTGTCTGCCCGACAGGGAGACGGCAGCCAGGTGGGC
      ********************************************************<<<<

  480 CACCAGATGCTTGTTAGCTGAGGGGCAGAAGCCAAGTTCTGGGCCTGGCTCTGATACCAA
      <<<<<<<<<<<<<<                                              

  540 GTAGCCTTGCAAGAGCCCCTTTCCCTTTTCCAGGCCTCGGTTTCTTGGAGTGAACCCAAA
                                                                  

  600 AGTTCTTTTCAGTACTGGCGTTTTATTTTTTATTTATATTTATTTATTTACTGACGGAGT
                                                                  

  660 TCCACTCTTGTCTCCCAGGCTGGAGTGTAGTTGTGCGATCTTGGCTCACTGCAACCCCAC
                                                                  

  720 CTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACA
                                                            

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4834     0   289     0     0     0     0   620  3010     0     0     0     0     0   915
Right   4762     0   289     0     0   176     0   739  2528     0     0     0    13     0  1017
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X12

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        280   18   59.97   55.56  2.00  0.00  9.00 CCCTTCTTCCTTCCCCAA
POSITION PENALTY, QUALITY: 0.000000, 2.027407
RIGHT PRIMER       515   18   62.87   66.67  3.00  2.00  8.00 CACTGTTCCCTCAGCCCG
POSITION PENALTY, QUALITY: 280.000000, 284.867800
SEQUENCE SIZE: 782
INCLUDED REGION SIZE: 782

PRODUCT SIZE: 236, PAIR ANY COMPL: 2.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,186

    0 CCACCTCCTGGGTTCAAGCGACTCTCCTGCCTCAGTCTCCTGAGTAGCTGGGATTACAGG
                                                                  

   60 CTAATTTTTGTATTTTTAGTAGAGACTGGTGGGTTTCACCGTGTCGGCCAGGTTGGTCTC
                                                                  

  120 GAACCCCTGACCTCAAGTGATTCACCCGCCTCGGCCTCCCAAAGTGCCGAGACCACAGGC
                                                                  

  180 GTGAACGTCTGTGCCCAGCCAGCTCTGGCGTTTTAGATTCTGGTCTCTAAGAAATGGCGT
                                                                  

  240 TGGGGCCAGGCGGCTCCTGTGGGGGTTGGCTCTCACTAGGCCCTTCTTCCTTCCCCAAAG
                                              >>>>>>>>>>>>>>>>>>**

  300 CTTGCTCCAGGCTGGATACAAGGGGCGGGTGACAGGCTGGGGCAACCTGAAGGAGACGTG
      ************************************************************

  360 GACAGCCAACGTTGGTAAGGGGCAGCCCAGTGTCCTGCAGGTGGTGAACCTGCCCATTGT
      ************************************************************

  420 GGAGCGGCCGGTCTGCAAGGACTCCACCCGGATCCGCATCACTGACAACATGTTCTGTGC
      ************************************************************

  480 TGGCAAGTCTGTGCAGGGCGGGCTGAGGGAACAGTGGGGCCCAAGCTGGGAGAACTGAGT
      ****              <<<<<<<<<<<<<<<<<<                        

  540 TGTGCCTGGGTTCAAGCCATGTGACTTTGAGCAAGTTGCCTAACCTCTTGGTGGCTCAGT
                                                                  

  600 TTCTTCCTCTGTAAAATGGAGGTAAAAGTCTCTATCCCATAAGGTTATGGGAGGGTTAAA
                                                                  

  660 TGAAGTAGTATATATTAATGTACTTGGCATAGTATCAGTCACCAGTGAGCTCAGATAGCA
                                                                  

  720 GCAAGAGGCTGCGGGTAGGGAAATGCCATTCATTCAGTCACTCAGCAAATATTTATTGAG
                                                                  

  780 CG
        

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4914     0   289     0     0    56     0   524  3341     0     0     0     0     0   704
Right   4914     0   289     0     0    83     0   976  2660     0     0     0     0     0   906
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X13

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   59.16   50.00  6.00  1.00  9.00 CAGACGGCTGTTCTCTTTCA
POSITION PENALTY, QUALITY: 0.000000, 0.838952
RIGHT PRIMER       394   19   59.02   57.89  7.00  2.00  9.00 GCCCTGGGCTTTAGAGAAG
POSITION PENALTY, QUALITY: 60.000000, 61.983042
SEQUENCE SIZE: 671
INCLUDED REGION SIZE: 671

PRODUCT SIZE: 117, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,75

    0 AACTTTTTAAAAGCATTATTCTGCCTGTTGGGTGGAGAATAGACTGTAGGTGGGCAAAGA
                                                                  

   60 ATGAAGGAAACTAGTGGGTTCAGGAGCTCGAGCTAGAAGTGGTGAGAAGGGTTTGGATTT
                                                                  

  120 GGGGTCTATGCTGAAGGTAGAGCCGACAAGATTTGCTAGGATTGGATGTGTAGGGTGAGG
                                                                  

  180 AAGTGGGGACAGCAAGAATGACTGGAGGGGTAAGTGGACTCTCACCAGCTGTGTCTCGTG
                                                                  

  240 AAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGCACAGACGGCTGTTCTCTTTCAAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 GTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTGTGAAGGTGACAGTGGGGGACCCT
      ************************************************************

  360 TTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCTGGTGAACACATCTTCTGGGGGTG
      *************   <<<<<<<<<<<<<<<<<<<                         

  420 GGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCAGAGGAATACTGATGTGACCTTGA
                                                                  

  480 ACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAGAGCCCCTTTAACAACCGCTGGTA
                                                                  

  540 TCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGACCGGGATGGGAAATATGGCTTCTA
                                                                  

  600 CACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAGGTCATTGATCAGTTTGGAGAGTA
                                                                  

  660 GGGGGCCACTC
                 

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3804     0   289     0     0    12     0   652  1841     0     0     0     0     0  1010
Right   3804     0   289     0     0     0     0   873  1715     0     5     0     0     0   922
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2_X14

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        274   24   60.11   37.50  5.00  0.00 10.00 TTGGAAACCTCATCTTTCTTCTTC
POSITION PENALTY, QUALITY: 0.000000, 4.108177
RIGHT PRIMER       466   20   59.66   55.00  4.00  2.00  9.00 GAGCCCAGAATATGAGTGGC
POSITION PENALTY, QUALITY: 20.000000, 20.341522
SEQUENCE SIZE: 685
INCLUDED REGION SIZE: 685

PRODUCT SIZE: 193, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,148

    0 ACTCTCACCAGCTGTGTCTCGTGAAGGGGCGTGGCTGGGCTATGAGCTATGCTCCTGAGC
                                                                  

   60 ACAGACGGCTGTTCTCTTTCAAGGTTACAAGCCTGATGAAGGGAAACGAGGGGATGCCTG
                                                                  

  120 TGAAGGTGACAGTGGGGGACCCTTTGTCATGAAGGTAAGCTTCTCTAAAGCCCAGGGCCT
                                                                  

  180 GGTGAACACATCTTCTGGGGGTGGGGAGAAACTCTAGTATCTAGAAACAGTTGCCTGGCA
                                                                  

  240 GAGGAATACTGATGTGACCTTGAACTTGACTCTATTGGAAACCTCATCTTTCTTCTTCAG
                                        >>>>>>>>>>>>>>>>>>>>>>>>**

  300 AGCCCCTTTAACAACCGCTGGTATCAAATGGGCATCGTCTCATGGGGTGAAGGCTGTGAC
      ************************************************************

  360 CGGGATGGGAAATATGGCTTCTACACACATGTGTTCCGCCTGAAGAAGTGGATACAGAAG
      ************************************************************

  420 GTCATTGATCAGTTTGGAGAGTAGGGGGCCACTCATATTCTGGGCTCCTGGAACCAATCC
      ************************** <<<<<<<<<<<<<<<<<<<<             

  480 CGTGAAAGAATTATTTTTGTGTTTCTAAAACTATGGTTCCCAATAAAAGTGACTCTCAGC
                                                                  

  540 GAGCCTCAATGCTCCCAGTGCTATTCATGGGCAGCTCTCTGGGCTCAGGAAGAGCCAGTA
                                                                  

  600 ATACTACTGGATAAAGAAGACTTAAGAATCCACCACCTGGTGCACGCTGGTAGTCCGAGC
                                                                  

  660 ACTCGGGAGGCTGAGGTGGGAGGAT
                               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4475     0   230     0     0     0     0   988  2162     0     0     0     0     0  1095
Right   3944     0   289     0     0    74     0   898  1848     0     2     0     0     0   833
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2R_X1

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        168   18   62.11   66.67  2.00  1.00  9.00 GAGGGTGAAGCGGAGCAG
POSITION PENALTY, QUALITY: 980.000000, 984.110493
RIGHT PRIMER       285   18   62.42   61.11  5.00  1.00  8.00 GCCGCACAGACTGAAGCA
POSITION PENALTY, QUALITY: 57000.000000, 57004.415904
SEQUENCE SIZE: 329
INCLUDED REGION SIZE: 329

PRODUCT SIZE: 118, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 235,90

    0 CTTCCTCGCTCGCGCCCGCGCGACCGCGCGCCCCAGTCCCGCCCCGCCCCGCTAACCGCC
                                                                  

   60 CCAGACACAGCGCTCGCCGAGGGTCGCTTGGACCCTGATCTTACCCGTGGGCACCCTGCG
                                                                  

  120 CTCTGCCTGCCGCGAAGACCGGCTCCCCGACCCGCAGAAGTCAGGAGAGAGGGTGAAGCG
                                                      >>>>>>>>>>>>

  180 GAGCAGCCCGAGGCGGGGCAGCCTCCCGGAGCAGCGCCGCGCAGAGCCCGGGACAATGGG
      >>>>>>                                                 *****

  240 GCCGCGGCGGCTGCTGCTGGTGGCCGCCTGCTTCAGTCTGTGCGGCCCGCTGTTGTCTGC
      ****************************((((((((((((((((((**************

  300 CCGCACCCGGGCCCGCAGGCCAGGTGAGA
      *************************    

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    2940     0     0     0     0   829     0    31  1984     0     0     0     0     0    96
Right    951     0   226     0     0   209     0     0   510     0     0     0     0     0     6
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2R_X2-seg1

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   23   58.00   26.09  5.00  2.00 11.00 TTTCAGAATCAAAAGCAACAAAT
POSITION PENALTY, QUALITY: 19000.000000, 19005.004751
RIGHT PRIMER       705   20   60.26   50.00  5.00  3.00 11.00 AGCAGGGTTTCATTGAGCAC
POSITION PENALTY, QUALITY: 20000.000000, 20000.262854
SEQUENCE SIZE: 706
INCLUDED REGION SIZE: 706

PRODUCT SIZE: 706, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 4,702

    0 TTTCAGAATCAAAAGCAACAAATGCCACCTTAGATCCCCGGTCATTTCTTCTCAGGAACC
      >>>>)))))))))))))))))))*************************************

   60 CCAATGATAAATATGAACCATTTTGGGAGGATGAGGAGAAAAATGAAAGTGGGTTAACTG
      ************************************************************

  120 AATACAGATTAGTCTCCATCAATAAAAGCAGTCCTCTTCAAAAACAACTTCCTGCATTCA
      ************************************************************

  180 TCTCAGAAGATGCCTCCGGATATTTGACCAGCTCCTGGCTGACACTCTTTGTCCCATCTG
      ************************************************************

  240 TGTACACCGGAGTGTTTGTAGTCAGCCTCCCACTAAACATCATGGCCATCGTTGTGTTCA
      ************************************************************

  300 TCCTGAAAATGAAGGTCAAGAAGCCGGCGGTGGTGTACATGCTGCACCTGGCCACGGCAG
      ************************************************************

  360 ATGTGCTGTTTGTGTCTGTGCTCCCCTTTAAGATCAGCTATTACTTTTCCGGCAGTGATT
      ************************************************************

  420 GGCAGTTTGGGTCTGAATTGTGTCGCTTCGTCACTGCAGCATTTTACTGTAACATGTACG
      ************************************************************

  480 CCTCTATCTTGCTCATGACAGTCATAAGCATTGACCGGTTTCTGGCTGTGGTGTATCCCA
      ************************************************************

  540 TGCAGTCCCTCTCCTGGCGTACTCTGGGAAGGGCTTCCTTCACTTGTCTGGCCATCTGGG
      ************************************************************

  600 CTTTGGCCATCGCAGGGGTAGTGCCTCTCGTCCTCAAGGAGCAAACCATCCAGGTGCCCG
      ************************************************************

  660 GGCTCAACATCACTACCTGTCATGATGTGCTCAATGAAACCCTGCT
      **************************((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    6710     0     0     0     0     4     0  1539  3376     0     0     1     0     0  1790
Right   6710     0     0     0     0     4     0  1540  3376     0     0     2     0     0  1788
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F2R_X2-seg2

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   20   59.50   45.00  5.00  1.00  9.00 TGATGTGCTCAATGAAACCC
POSITION PENALTY, QUALITY: 20000.000000, 20000.498267
RIGHT PRIMER       532   19   59.79   57.89  2.00  0.00  9.00 CTCCCAGCAGTCCCTTTTC
POSITION PENALTY, QUALITY: 0.000000, 1.212099
SEQUENCE SIZE: 750
INCLUDED REGION SIZE: 750

PRODUCT SIZE: 533, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 0,514

    0 TGATGTGCTCAATGAAACCCTGCTCGAAGGCTACTATGCCTACTACTTCTCAGCCTTCTC
      ))))))))))))))))))))****************************************

   60 TGCTGTCTTCTTTTTTGTGCCGCTGATCATTTCCACGGTCTGTTATGTGTCTATCATTCG
      ************************************************************

  120 ATGTCTTAGCTCTTCCGCAGTTGCCAACCGCAGCAAGAAGTCCCGGGCTTTGTTCCTGTC
      ************************************************************

  180 AGCTGCTGTTTTCTGCATCTTCATCATTTGCTTCGGACCCACAAACGTCCTCCTGATTGC
      ************************************************************

  240 GCATTACTCATTCCTTTCTCACACTTCCACCACAGAGGCTGCCTACTTTGCCTACCTCCT
      ************************************************************

  300 CTGTGTCTGTGTCAGCAGCATAAGCTCGTGCATCGACCCCCTAATTTACTATTACGCTTC
      ************************************************************

  360 CTCTGAGTGCCAGAGGTACGTCTACAGTATCTTATGCTGCAAAGAAAGTTCCGATCCCAG
      ************************************************************

  420 CAGTTATAACAGCAGTGGGCAGTTGATGGCAAGTAAAATGGATACCTGCTCTAGTAACCT
      ************************************************************

  480 GAATAACAGCATATACAAAAAGCTGTTAACTTAGGAAAAGGGACTGCTGGGAGGTTAAAA
      **********************************<<<<<<<<<<<<<<<<<<<       

  540 AGAAAAGTTTATAAAAGTGAATAACCTGAGGATTCTATTAGTCCCCACCCAAACTTTATT
                                                                  

  600 GATTCACCTCCTAAAACAACAGATGTACGACTTGCATACCTGCTTTTTATGGGAGCTGTC
                                                                  

  660 AAGCATGTATTTTTGTCAATTACCAGAAAGATAACAGGACGAGATGACGGTGTTATTCCA
                                                                  

  720 AGGGAATATTGCCAATGCTACAGTAATAAA
                                    

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4999     0   227     0     0     0     0  1458  1982     0     0     0    22     0  1310
Right   6997     0     0     0     0   109     0  2483  2360     0     0     0    22     0  2023
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F3_X1

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        280   18   62.92   66.67  4.00  2.00  8.00 CTCGCCGCCAACTGGTAG
POSITION PENALTY, QUALITY: 0.000000, 4.915955
RIGHT PRIMER       459   18   61.41   66.67  3.00  1.00  8.00 ACTGTCGCCTCCCTCCTG
POSITION PENALTY, QUALITY: 800.000000, 803.413051
SEQUENCE SIZE: 700
INCLUDED REGION SIZE: 700

PRODUCT SIZE: 180, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,104

    0 ACCGGGAGGAGGCGGGGCAGGGGTGTGGACTCGCCGGGGGCCGCCCACCGCGACGGCAAG
                                                                  

   60 TGACCCGGGCCGGGGGCGGGGAGTCGGGAGGAGCGGCGGGGGCGGGCGCCGGGGGCGGGC
                                                                  

  120 AGAGGCGCGGGAGAGCGCGCCGCCGGCCCTTTATAGCGCGCGGGGCACCGGCTCCCCAAG
                                                                  

  180 ACTGCGAGCTCCCCGCACCCCCTCGCACTCCCTCTGGCCGGCCCAGGGCGCCTTCAGCCC
                                                                  

  240 AACCTCCCCAGCCCCACGGGCGCCACGGAACCCGCTCGATCTCGCCGCCAACTGGTAGAC
                                              >>>>>>>>>>>>>>>>>>**

  300 ATGGAGACCCCTGCCTGGCCCCGGGTCCCGCGCCCCGAGACCGCCGTCGCTCGGACGCTC
      ************************************************************

  360 CTGCTCGGCTGGGTCTTCGCCCAGGTGGCCGGCGCTTCAGGTGAGTGGCACCAGCCCCTG
      ******************************************                  

  420 GAAGCCCGGGGCGCGCCACACGCAGGAGGGAGGCGACAGTCCTGGCTGGCAGCGGGCTCG
                            <<<<<<<<<<<<<<<<<<                    

  480 CCCTGGTTCCCCGGGGCGCCCATGTTGTCCCCCGCGCCTACGGGACTCGGCTGCGCTCAC
                                                                  

  540 CCAGCCCGGCTTGAATGAACCGAGTCCGTCGGGCGCCGGCGGGAGTTGCAGGGAGGGAGT
                                                                  

  600 TGGCGCCCCAGACCCCGCTGCCCCTTCCGCTGGAGAGTTTTGCTCGGGGTGTCCGAGTAA
                                                                  

  660 TTGGACTGTTGTTGCATAAGCGGACTTTTAGCTCCCGCTT
                                              

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4094     0   289     0     0  1473     0    23  2263     0     0     0     0     0    46
Right   4094     0   289     0     0   716     0   101  2783     0     0     0     0     0   205
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F3_X2

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        266   27   57.78   33.33  6.00  0.00 11.00 CATGTAATAATAGCTGTTTCTCTGTTG
POSITION PENALTY, QUALITY: 100.000000, 109.223369
RIGHT PRIMER       436   23   58.56   34.78  4.00  2.00 11.00 TTTTCTTTTTCTGTACCCAGCTT
POSITION PENALTY, QUALITY: 0.000000, 4.440107
SEQUENCE SIZE: 712
INCLUDED REGION SIZE: 712

PRODUCT SIZE: 171, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,116

    0 CTCTGGGCTCTTCCCGGTCAAGTTATAACAAGGCTTTGCCCATGAATAATTTCAAACGAA
                                                                  

   60 AATGTCAAGATCCTTGCCGGTGTCCTGGGATTACAAGGTGAATCTTGTCATGAAGAAATT
                                                                  

  120 CTAGGTCTAGAAAAAATTTGAAGATTCTTTTTCTCTTGATAATTCACTAATGAAGCTTTT
                                                                  

  180 GTGGTTGAAAAATAAAAAGTGAGGTTTATGGTGATGTCAGGTGGGAAGGTGTTTTATACA
                                                                  

  240 TCAATACATTCGAGTGCTCTGAAGTGCATGTAATAATAGCTGTTTCTCTGTTGTTTAAAG
                                >>>>>>>>>>>>>>>>>>>>>>>>>>>     **

  300 GCACTACAAATACTGTGGCAGCATATAATTTAACTTGGAAATCAACTAATTTCAAGACAA
      ************************************************************

  360 TTTTGGAGTGGGAACCCAAACCCGTCAATCAAGTCTACACTGTTCAAATAAGGTAAGCTG
      ******************************************************<<<<<<

  420 GGTACAGAAAAAGAAAATTAAGGTCTTTGATGTTTCTACTGTCCTATGCTGAACAAGAAT
      <<<<<<<<<<<<<<<<<                                           

  480 GTCTTTAAAGCTGATTACTGGATGAAATTATTTAACAGATGACGAAGAAGAAGGGATTCT
                                                                  

  540 TGGCAATTCGCTGGCCGGTGTCATACTCTATTAGGCCTGCAACATTTCCAGACCTTAAAC
                                                                  

  600 TGATAGAACATTTTAATTGTTTTAATTGTTTTTGGAAATGATGGGAGAGTTCCTAAGTGG
                                                                  

  660 AGTATAAACTGTGGAGAGATGAACCATCTTGAGTAGGCACTGAAGTGTGCTT
                                                          

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4082     0   289     0     0    83     0  1878   779     0     0     1    20     0  1032
Right   4214     0   289     0     0   232     0  1968   682     0     0     0     0     0  1043
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F3_X3

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        264   24   57.53   29.17  4.00  0.00 11.00 TTCCATTTATGTTTCTTCCTTCTT
POSITION PENALTY, QUALITY: 200.000000, 206.472471
RIGHT PRIMER       521   20   57.40   45.00  3.00  3.00  9.00 TATTACAGCCCAAGCCACTT
POSITION PENALTY, QUALITY: 0.000000, 2.604792
SEQUENCE SIZE: 800
INCLUDED REGION SIZE: 800

PRODUCT SIZE: 258, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,204

    0 TGAGGACTCAAACATGCATCCCAAGTGCTCGGTGTTGCTAGGTATGATGGCTCACACCTG
                                                                  

   60 TACATTCAGCACTTTGGGAGGCCGAAGCAGAAGGATCAGCCTGGGCAACATAGCAGGACC
                                                                  

  120 CCATCTCTACAAAACAATGTTTAAAAAAAAGCAAAGTGCTCAGCACAGTGACTGCATCAT
                                                                  

  180 TAGGATTGATTGTAGGGCTCCTGATGTTAGCACAGAACACCACAGCCAGGAAGCAGTCTA
                                                                  

  240 TCTTGTTGGGTGCAAATTGTAACATTCCATTTATGTTTCTTCCTTCTTTTCTTTCTTTAG
                              >>>>>>>>>>>>>>>>>>>>>>>>          **

  300 CACTAAGTCAGGAGATTGGAAAAGCAAATGCTTTTACACAACAGACACAGAGTGTGACCT
      ************************************************************

  360 CACCGACGAGATTGTGAAGGATGTGAAGCAGACGTACTTGGCACGGGTCTTCTCCTACCC
      ************************************************************

  420 GGCAGGGAATGTGGAGAGCACCGGTTCTGCTGGGGAGCCTCTGTATGAGAACTCCCCAGA
      ************************************************************

  480 GTTCACACCTTACCTGGAGAGTAAGTGGCTTGGGCTGTAATACCGTTCATTCTTGTTAGA
      **********************<<<<<<<<<<<<<<<<<<<<                  

  540 AACGTCTGAACATTCTCGTGATCTTGTGCCTTTAGGGGCTACAAAATTAAAAATATTTAT
                                                                  

  600 TCTTTTTTTCTCAGAAACTGGTATGTATCACAGCCCTCTTCACACATTCCAGATGTGGTA
                                                                  

  660 GGAGGTTCACAGAATGTGAACTTTTGGAGCTGATGACAGTGTCATCAAGTAACTTTCTCC
                                                                  

  720 CCCAGTCTGTCCCCAGACCCTGTTACTGTCCTCAGTAAGCGGCTGAATGTGTGTTGGGAG
                                                                  

  780 AGGGCGGGCCAGGGAAGCGG
                          

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4965     0   289     0     0    54     0  1212  2106     0     0    28    22     0  1254
Right   5005     0   289     0     0   185     0  1231  1945     0     0     0    11     0  1344
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F3_X4

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        276   22   60.11   40.91  4.00  0.00 11.00 CCAAGCTCATTTCCTTTGTTTC
POSITION PENALTY, QUALITY: 0.000000, 2.108929
RIGHT PRIMER       523   27   58.95   25.93  6.00  1.00 10.00 TCACAATTTAAAACAGGTCATAAAAAC
POSITION PENALTY, QUALITY: 320.000000, 328.049552
SEQUENCE SIZE: 779
INCLUDED REGION SIZE: 779

PRODUCT SIZE: 248, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,183

    0 TGGTTAAATAAAATATATTATTAAAATTAATTTCACCTGTTCCTTTTTACTTTTTCTAAT
                                                                  

   60 GTGACTACTAGAAAACTTAAAATGACATCTGAGGCTCCATTGTCTTCCCCTTGGGCCAGC
                                                                  

  120 ACTACCACAGAATGTCTTAGGATTCAGCTCCAGGCCGCCACGCCTGCTTCTTTCAGGGAG
                                                                  

  180 CTGGTTCTATGCACATGTTTTATATGAGAGATAATTAAGTTGTCAATTGTGATAACAAAA
                                                                  

  240 CAGGATTTGACTTTGTACAGAATTCTTTGGTTCCAACCAAGCTCATTTCCTTTGTTTCAG
                                          >>>>>>>>>>>>>>>>>>>>>>**

  300 CAAACCTCGGACAGCCAACAATTCAGAGTTTTGAACAGGTGGGAACAAAAGTGAATGTGA
      ************************************************************

  360 CCGTAGAAGATGAACGGACTTTAGTCAGAAGGAACAACACTTTCCTAAGCCTCCGGGATG
      ************************************************************

  420 TTTTTGGCAAGGACTTAATTTATACACTTTATTATTGGAAATCTTCAAGTTCAGGAAAGG
      ************************************************************

  480 TGAGCATTTTTTAATTTGTTTTTATGACCTGTTTTAAATTGTGAATACTTGGTTTTACAA
      *                <<<<<<<<<<<<<<<<<<<<<<<<<<<                

  540 CCCATTTCTTCCCCAATTCAAAAATAGCAGAACAGAGTTGTTGAGAAGGTGATGGAGTAG
                                                                  

  600 AAGGGGGAGCGCGCACTGTGGGGAGGGGTGGACAACAGGCCTGGTCCTACCTGTGACTCT
                                                                  

  660 GCACTACCCTGTGACTCTGGCAGGGCCCCCTCGGAGACCCAGGTTCCTCAGCCAACCGGC
                                                                  

  720 TGGATCAGGTCATCTCTAAAGGTCCCGCCACGCTCACATTTCTCCCTCTATTGAGGATC
                                                                 

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4884     0   289     0     0   320     0  1911  1322     0     6     0     0     0  1036
Right   4770     0   289     0     0   176     0  1330  1913     0     0     0    21     0  1041
Pair Stats:
considered 36, high end compl 33, ok 3
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F3_X5

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        268   27   57.07   29.63  7.00  1.00 10.00 CCAGATGAATTAAGAACTCTATCTTTT
POSITION PENALTY, QUALITY: 60.000000, 69.931898
RIGHT PRIMER       485   18   62.91   61.11  5.00  1.00  9.00 GGCAAATGGCTGGCAGAG
POSITION PENALTY, QUALITY: 120.000000, 124.906952
SEQUENCE SIZE: 760
INCLUDED REGION SIZE: 760

PRODUCT SIZE: 218, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,164

    0 CAAAATTTGTTTTTGGTTCAATGCATAATACTCCCTTCCTTTTTCTTTTACTGCAGATAT
                                                                  

   60 CTTCTAAAGGGGCTCAATAGGGTTCAATATGCCTAAATTGGATCTTCTCAGTCTTGGAAA
                                                                  

  120 AGGCATTTTTAGCAGTGATCAAGGGAAACTGATTAGCGAAGTCACTTCTAATCCTTCACG
                                                                  

  180 TGTCAGCTGTGTTCTTGTAGGCTTTGCTTAGAACCTAGGTTTTTACTTCCACAGTGACTT
                                                                  

  240 AATAAAGGGGAAAGAATTGACTCAGAGCCCAGATGAATTAAGAACTCTATCTTTTTACAG
                                  >>>>>>>>>>>>>>>>>>>>>>>>>>>   **

  300 AAAACAGCCAAAACAAACACTAATGAGTTTTTGATTGATGTGGATAAAGGAGAAAACTAC
      ************************************************************

  360 TGTTTCAGTGTTCAAGCAGTGATTCCCTCCCGAACAGTTAACCGGAAGAGTACAGACAGC
      ************************************************************

  420 CCGGTAGAGTGTATGGGCCAGGAGAAAGGGGAATTCAGAGGTGAGTGGCTCTGCCAGCCA
      ******************************************      <<<<<<<<<<<<

  480 TTTGCCTGGGGGTATGGGTGCTGTGGGTGACTTCTGGAGGAGTAGCTCCACCCTCAGGGC
      <<<<<<                                                      

  540 TGGGATATACTTCCTTGGTTAAATATTCAGGAAAACAAACTGCCTGGAGGTTTTTTGTTG
                                                                  

  600 TTATTTGTTTGTTTTGGTTTTGATTTTGCTTTGGTACAAAAAAGATTTTGGACATTTAGA
                                                                  

  660 AATGTTTCTGTGTTGATTGTGCCCTTGTATTAGCAGGTGTTTTCTTGAGCACCTGTCATG
                                                                  

  720 TGCTAAGCCCTCTGCTGAGCACTGGATACACAAACTGTGT
                                              

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4694     0   289     0     0     4     0  2035   844     0     0     0     0     0  1522
Right   4397     0   289     0     0    28     0  1323  1565     0     4     0    44     0  1144
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F3_X6

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        274   24   58.01   33.33  4.00  0.00 11.00 TTGACTAGATGCCCTTTATTTTTC
POSITION PENALTY, QUALITY: 0.000000, 5.991812
RIGHT PRIMER       459   21   57.77   52.38  4.00  1.00 11.00 CAGTAGCTCCAACAGTGCTTC
POSITION PENALTY, QUALITY: 0.000000, 3.226865
SEQUENCE SIZE: 737
INCLUDED REGION SIZE: 737

PRODUCT SIZE: 186, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,141

    0 CAGCATCGTTTTAGGCACTTCTTTCCAGCCATTGTTCTTACCTCCAACTACATATCTTTT
                                                                  

   60 CTGGACTGTGCATTATTCAGTTTATTAAATGCCCATTAAATGTGTTTAGCCATTGTCAAT
                                                                  

  120 TACTCTGAAACGTTCAGGTTTTGACAAATTCTTTCCTAATGTAAGTGTGGTGGAAAGAGT
                                                                  

  180 GAAAGAAAGTCAAATTGCACAAAAATAGGATGGTGTAATTTGGGGTTATGCCGTCAATTT
                                                                  

  240 TGTCCACTGATAAATGGGATTTGAGCTCTCCAAGTTGACTAGATGCCCTTTATTTTTCAG
                                        >>>>>>>>>>>>>>>>>>>>>>>>**

  300 AAATATTCTACATCATTGGAGCTGTGGTATTTGTGGTCATCATCCTTGTCATCATCCTGG
      ************************************************************

  360 CTATATCTCTACACAAGTGTAGAAAGGCAGGAGTGGGGCAGAGCTGGAAGGAGAACTCCC
      ************************************************************

  420 CACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTATATTGCACTGT
      *******************<<<<<<<<<<<<<<<<<<<<<                    

  480 GACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCATG
                                                                  

  540 AAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTT
                                                                  

  600 TTGACATCAGCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTT
                                                                  

  660 TTAATTTTTAACACCATGGCACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCA
                                                                  

  720 CTCAAGGAGTAACCAGG
                       

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4464     0   289     0     0    57     0  1831   878     0     0     0     0     0  1409
Right   4311     0   289     0     0    51     0  1766   895     0     0     0    22     0  1288
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F5_X10

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER         23   23   59.52   21.74  6.00  3.00 10.00 TTGCAAATGAAAACAATTTTGAA
POSITION PENALTY, QUALITY: 260.000000, 263.479042
RIGHT PRIMER       302   24   59.24   33.33  5.00  0.00 10.00 TGAAAGGTTACTTCAAGGACAAAA
POSITION PENALTY, QUALITY: 20.000000, 24.758462
SEQUENCE SIZE: 319
INCLUDED REGION SIZE: 319

PRODUCT SIZE: 280, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 59,219

    0 TTATTTATTATCATGAAATAACTTTGCAAATGAAAACAATTTTGAATATATTTTCTTTCA
                             >>>>>>>>>>>>>>>>>>>>>>>             *

   60 GGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGG
      ************************************************************

  120 AACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATAC
      ************************************************************

  180 TACAGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATC
      ************************************************************

  240 TGTAAGAGCAGATCCCTGGACAGGCGAGGAATACAGGTATTTTGTCCTTGAAGTAACCTT
      ************************************** <<<<<<<<<<<<<<<<<<<<<

  300 TCAGAAATTCTGAGAATTT
      <<<                

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    2597     0    32     0     0   195     0  1010   658     0    23     8     0     0   671
Right   2435     0    50     0     0     0     0  1068   632     0     0     0     0     0   685
Pair Stats:
considered 18, high end compl 16, ok 2
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F7_X1

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   60.30   50.00  6.00  2.00  8.00 GCAGCACTGCAGAGATTTCA
POSITION PENALTY, QUALITY: 0.000000, 0.295503
RIGHT PRIMER       385   18   59.44   61.11  4.00  2.00  7.00 GAGAAAACCTCCCCGGAC
POSITION PENALTY, QUALITY: 40.000000, 42.564912
SEQUENCE SIZE: 664
INCLUDED REGION SIZE: 664

PRODUCT SIZE: 108, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,68

    0 ATTTGAAGTGTTGGTGCCACACACACCTTAACACCTGCACGCTGGCAACAAAACCGTCCG
                                                                  

   60 CTCTGCAGCACAGCTGGGGTCACCTGACCTTTCTCCTGTCCCCCCCACTTGAGCTCAGTG
                                                                  

  120 GCTGGGCAGCAGGGGATGCATGGCCACTGGCGGCCAGGTGCAGCTCTCAGCTGGGGTGTT
                                                                  

  180 CAGAGGACGCCTGTGTCCTCCCCTCCCCCATCCCTCTGTCACCCTTGGAGGCAGAGAACT
                                                                  

  240 TTGCCCGTCAGTCCCATGGGGAATGTCAACAGGCAGGGGCAGCACTGCAGAGATTTCATC
                                            >>>>>>>>>>>>>>>>>>>>**

  300 ATGGTCTCCCAGGCCCTCAGGCTCCTCTGCCTTCTGCTTGGGCTTCAGGGCTGCCTGGCT
      ************************************************************

  360 GCAGGTGCGTCCGGGGAGGTTTTCTCCATAAACTTGGTGGAAGGGCAGTGGGCAAATCCA
      ******  <<<<<<<<<<<<<<<<<<                                  

  420 GGAGCCAGCCCGGGCTTCCCAAACCCCGCCCTTGCTCCGGACACCCCCATCCACCAGGAG
                                                                  

  480 GGTTTTCTGGCGGCTCCTGTTCAATTTCTTTCCTTCTAGAAACCAGCATCCAGGCACAGG
                                                                  

  540 AGGGGAGGCCCTTCTTGGTAGCCCAGGCTTTGGCGGGATTATTTTTCAAAGAACTTTAGG
                                                                  

  600 AGTGGGTGGTGCTTTCCTGGCCCCCATGGCCCTGCCTGTGAGGTCGGACAAGCGCAGGGA
                                                                  

  660 GTCT
          

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3572     0   289     0     0     0     0   118  2723     0     0     0    23     0   419
Right   3734     0   289     0     0    17     0   289  2735     0     1     0     0     0   403
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F7_X2

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        265   18   62.24   61.11  4.00  2.00  8.00 CATGGCCTTATGACCCCG
POSITION PENALTY, QUALITY: 300.000000, 304.241567
RIGHT PRIMER       385   18   60.58   66.67  5.00  3.00  9.00 CAGTGGCAGTCCCTGCTC
POSITION PENALTY, QUALITY: 0.000000, 2.576172
SEQUENCE SIZE: 666
INCLUDED REGION SIZE: 666

PRODUCT SIZE: 121, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,70

    0 TGTGTGGGGTGTGGGGATAGGGTCAGGGGATGGCGTGGGGGGTGTGGCATGGGGATGGCA
                                                                  

   60 CGTGTGGCATGGGGATGGGGATGGGGGGTGGGGCATGGCCGAGTGGGGCTGGGGCTGGGA
                                                                  

  120 ATGGTGAGTGGGGCATGGGGATGGCGAGTAGGGGGTGTGGCGTGAGGATGGCTAGTGGGG
                                                                  

  180 CGTGGGGATGGCGTGTGGGGATGGCGAGTGGGGGGTGGGCTGTGAGGGACAGTGCCTGGG
                                                                  

  240 ATGTGGCTGCAGCCCTAGCTCACAGCATGGCCTTATGACCCCGGCCACCTTCCTGCCCAG
                               >>>>>>>>>>>>>>>>>>               **

  300 GCGGGGTCGCTAAGGCCTCAGGAGGAGAAACACGGGACATGCCGTGGAAGCCGGGGCCTC
      ************************************************************

  360 ACAGAGGTGAGCAGGGACTGCCACTGGTTTTGTCCTGGGGCCCAGTGGGGGCAACATCAC
      ********<<<<<<<<<<<<<<<<<<                                  

  420 CTCCTTCCCCTCCCATGGCAAAGAGCCAGCCCGCGGGGTGGCTACTGCAGTGCCCCCCAA
                                                                  

  480 GGAGGGTGTTCCCTGCTCGAGAGGAAGTGACCGCTCCAGCTTGGCCTTCCCTGGGACTGG
                                                                  

  540 GGTGCAGGCGATTTTATCTTCTTTGCTCCATTCTGTTCCTTCCAGATAATCGTGTGTTCT
                                                                  

  600 TCATCAGGTTTTCCTCAGTTCTTGAGAGCTTTTCTGATGCAAATCTGCTTTCACCCCAGG
                                                                  

  660 GCGGTC
            

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3301     0   289     0     0    28     0    14  2710     0     1     0    65     0   194
Right   3601     0   289     0     0    16     0   390  2278     0     0     0    22     0   606
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F7_X2'

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        276   19   58.86   47.37  4.00  2.00  9.00 CTTCACGGAACTCGCATTT
POSITION PENALTY, QUALITY: 60.000000, 62.138031
RIGHT PRIMER       546   18   61.66   61.11  3.00  2.00  9.00 AGCCAAAGAGACGCAGGC
POSITION PENALTY, QUALITY: 1320.000000, 1323.664208
SEQUENCE SIZE: 761
INCLUDED REGION SIZE: 761

PRODUCT SIZE: 271, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,165

    0 AGCCTCACGTTTACCGCGGCGCCCGCAGCCCCCTTCGCCCGCTTCCGCGCGTGCCCCCGA
                                                                  

   60 GCGCGCCCTCGGGATCAGCCCCCGGAAGCAGAGAGGCCAGGCCGGGAAGGATGGGCGAAC
                                                                  

  120 GGGGTGGCTGACCCGGGAGCACGGCAGGGAGGACACCCAGCCAGGCCCGCGAGCAGCGCC
                                                                  

  180 GCTCCCCTCCTCCAGGACGGGCGGGAACCTGCGATGCCCCCGCCGCGTGGGCCGTGGGGC
                                                                  

  240 GGTCTCCGAGGCACTGGGCGGGGCACGCGGTGGGCGCTTCACGGAACTCGCATTTCCCAG
                                          >>>>>>>>>>>>>>>>>>>   **

  300 TCTTCGTAACCCAGGAGGAAGCCCACGGCGTCCTGCACCGGCGCCGGCGCGCCAACGCGT
      ************************************************************

  360 TCCTGGAGGAGCTGCGGCCGGGCTCCCTGGAGAGGGAGTGCAAGGAGGAGCAGTGCTCCT
      ************************************************************

  420 TCGAGGAGGCCCGGGAGATCTTCAAGGACGCGGAGAGGACGGTGAGCCCAGCCTCGGGGC
      *******************************************                 

  480 GCCCCGCGCGGACACTGCACGGCGGCGGTGAACCAGGCCGCGTGGGGCCGCCTGCGTCTC
                                                       <<<<<<<<<<<

  540 TTTGGCTGCGGCCTGTGGGCGGCGAACACGCAGCGGCGCCCGCGCGCGCGCTCTCTCTGC
      <<<<<<<                                                     

  600 GGGGGTCGCTTTCCGCCCGGGGTGACTCCGCTTTCCTGGGCGATGCCCCCACCCCCAGGC
                                                                  

  660 ACGCGCTCTCCCCGTGCGGCCGCACCGCGCATGCCGGTTTTCACATCAGAAAATACGATT
                                                                  

  720 TGCACAAGCACACTTAGGGTGTCCCCCTTAACTTCCCAAGG
                                               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4704     0   289     0     0  1097     0    35  3097     0     6     0     0     0   180
Right   4704     0   289     0     0  1078     0   138  2874     0     0     0     0     0   325
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F7_X3

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        277   21   60.51   52.38  2.00  0.00 10.00 GGCTTGCTCTCTTGTTCCTTC
POSITION PENALTY, QUALITY: 0.000000, 1.510814
RIGHT PRIMER       359   18   59.83   61.11  4.00  2.00 11.00 GTTGCAGGCAGGACTGGT
POSITION PENALTY, QUALITY: 300.000000, 302.172769
SEQUENCE SIZE: 625
INCLUDED REGION SIZE: 625

PRODUCT SIZE: 83, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,29

    0 CAGAGCCTGTGTACCTCGTCCCAGGTCCACAGCTCAGCGACAGAAGAGTCAGGGTTGAAC
                                                                  

   60 CTCGGGTGTTCTGACTTGGGAGCAGGAAATGTGTGGTCACCCATAGTTCCAGATGTCCTG
                                                                  

  120 GGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGCTCCAGAGGAAAGTCTGGCTTCCTGA
                                                                  

  180 GCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAACCCCAGTTCATGGTGTGTCCAGTGC
                                                                  

  240 TTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCACGAGGCTTGCTCTCTTGTTCCTTCAG
                                           >>>>>>>>>>>>>>>>>>>>>**

  300 AAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGATGATCACCACCAGTCCTGCCTGCAAC
      ***************************               <<<<<<<<<<<<<<<<<<

  360 CCTTCTCAGCTTACTGACACCAGCCCACTCCACAGATGGGGACCAGTGTGCCTCAAGTCC
                                                                  

  420 ATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCCAGTCCTATATCTGCTTCTGCCTCCC
                                                                  

  480 TGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGGCCCCACTTTGGGTCCCATATTTGCA
                                                                  

  540 GAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCCAACCGGGCTGCAGGGTGCAACAACC
                                                                  

  600 TGGTGGGGTGTGTAGGCCGGGCATT
                               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3344     0   289     0     0     2     0   424  1910     0     0     0     0     0   719
Right   3344     0   289     0     0     0     0   284  2312     8    13     7     0     0   431
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F7_X4

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        280   18   60.10   66.67  2.00  0.00 10.00 GACACCAGCCCACTCCAC
POSITION PENALTY, QUALITY: 0.000000, 2.096539
RIGHT PRIMER       433   18   62.23   61.11  5.00  1.00  8.00 GACCCAAAGTGGGGCCTT
POSITION PENALTY, QUALITY: 0.000000, 4.227266
SEQUENCE SIZE: 714
INCLUDED REGION SIZE: 714

PRODUCT SIZE: 154, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,118

    0 GTCACCCATAGTTCCAGATGTCCTGGGGAGGGGCCAAGATTAGAAGAAACCTACCTCAGC
                                                                  

   60 TCCAGAGGAAAGTCTGGCTTCCTGAGCCCACCCCGCCAGACCCAGGTCCAAGTCCCCCAA
                                                                  

  120 CCCCAGTTCATGGTGTGTCCAGTGCTTACCGTTGGGTGCTCTGGTGAAGGTGCATCTCAC
                                                                  

  180 GAGGCTTGCTCTCTTGTTCCTTCAGAAGCTGTTCTGGATTTCTTACAGTGGTGAGTGGAT
                                                                  

  240 GATCACCACCAGTCCTGCCTGCAACCCTTCTCAGCTTACTGACACCAGCCCACTCCACAG
                                              >>>>>>>>>>>>>>>>>>**

  300 ATGGGGACCAGTGTGCCTCAAGTCCATGCCAGAATGGGGGCTCCTGCAAGGACCAGCTCC
      ************************************************************

  360 AGTCCTATATCTGCTTCTGCCTCCCTGCCTTCGAGGGCCGGAACTGTGAGACGCGTAAGG
      ********************************************************<<<<

  420 CCCCACTTTGGGTCCCATATTTGCAGAGGGCCCTGGGGAGCTGGTGGAGGTGGCCTGGCC
      <<<<<<<<<<<<<<                                              

  480 AACCGGGCTGCAGGGTGCAACAACCTGGTGGGGTGTGTAGGCCGGGCATTCAGGGCTCAG
                                                                  

  540 CCCAGTTGGAAATTGGTCTAGGTGACCTTTAAATCCCTTCCAGTCTGAGGTCTTTGACAG
                                                                  

  600 GGACCCAAGGTTCTGATTATCAGACTCAGTGGCCCCCTTCGCGGTCCCGGCCCTGGGCAA
                                                                  

  660 CTTCTCAGCCCTGGAGACTGGCCCAGTTGAGAGTCCCTGTGTCCCGTGTGCCCA
                                                            

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4234     0   289     0     0     2     0   537  2588     0     0     0     0     0   818
Right   4234     0   289     0     0    81     0   378  2891     0     0     0     0     0   595
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F7_X5

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        277   18   60.31   66.67  3.00  1.00  9.00 GGGTGTCCCCTTCCTGTC
POSITION PENALTY, QUALITY: 60.000000, 62.311131
RIGHT PRIMER       461   18   60.05   55.56  4.00  1.00 11.00 TGGGACATGAAGCCTGGT
POSITION PENALTY, QUALITY: 20.000000, 22.045801
SEQUENCE SIZE: 741
INCLUDED REGION SIZE: 741

PRODUCT SIZE: 185, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,145

    0 GCTCGGAAGAGTGGTCACCCTGGAGGCCACTGCCCACCAGCCAGGCACCCCCCAAATGCA
                                                                  

   60 ACCGCAGCCAGCACTGCCAGCCACTGGCAAGGCTGTTCAGACATGTGGCTCCTCTGATCC
                                                                  

  120 ACGCCTTGTCCTTTGGATCAGTCCACGGAGCAGTGTGCCAAGCTCAGGCTCTGTCACCCA
                                                                  

  180 CAGCTCATGCCACCTTCCAGGCAGAACACCACTGCTGACCCAGGGGCATGGCCACCCCGG
                                                                  

  240 GGGCTGGCGTCTCGCTGACCCCCAGAAGCCCCTCTCAGGGTGTCCCCTTCCTGTCCCCAG
                                           >>>>>>>>>>>>>>>>>>   **

  300 ACAAGGATGACCAGCTGATCTGTGTGAACGAGAACGGCGGCTGTGAGCAGTACTGCAGTG
      ************************************************************

  360 ACCACACGGGCACCAAGCGCTCCTGTCGGTGCCACGAGGGGTACTCTCTGCTGGCAGACG
      ************************************************************

  420 GGGTGTCCTGCACACCCACAGGTGACCAGGCTTCATGTCCCAGTCCCAGATGACACCAGT
      *********************** <<<<<<<<<<<<<<<<<<                  

  480 CCCTGTCCCACTAGGATTATCTTACTGGACAAAAGACGGGTGGGACTGGCCTTCACATCT
                                                                  

  540 ACTGAGCACTAACTATGCACTGACCAATTGTGAGGTGGGATCTGGGCACCAAGGGTGGCA
                                                                  

  600 CAGGCCAGCAGCGACCAGTGACTAGGATGGGCACCCTGGGGGCAATCCCTGAATGGCCTC
                                                                  

  660 AGGCCCCCTGCCAACTTCTAGGCAGACCAGGGGAGCCAAGCAAGGCACTATCTCACGTCC
                                                                  

  720 AACTGCCCACTCGCAGGAATC
                           

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4351     0   289     0     0   139     0   152  3259     0     0     0    22     0   490
Right   4504     0   289     0     0     1     0   499  3030     0     0     2     0     0   683
Pair Stats:
considered 20, high any compl 8, high end compl 10, ok 2
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F7_X6

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        272   26   59.68   34.62  3.00  3.00  9.00 GCATCTTTCTGACTTTTGTTTTACAC
POSITION PENALTY, QUALITY: 0.000000, 6.324793
RIGHT PRIMER       434   19   61.51   57.89  4.00  0.00  8.00 ATCCTGAAGCCAGGGGAAG
POSITION PENALTY, QUALITY: 80.000000, 82.512614
SEQUENCE SIZE: 710
INCLUDED REGION SIZE: 710

PRODUCT SIZE: 163, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,114

    0 GAAAAGTGAGCAGAGGGCGGTTTTAAGACGTAAGCCCTCTGTTTCCTCCAAAACCAGCCC
                                                                  

   60 TGACCATTGTCTCCTCAGCCAGCCACTTCTTCAAGGGCCTCTCATGGCCGGGCCCCACCA
                                                                  

  120 GTCAGGCCCAGCCGAGGCCCTGCCTTCCACCACCCCTGGGCCCTGGGAGCTCCTGCTCCT
                                                                  

  180 GGGGGCCTCCCATAGCCTCGGCCTCAAGGCCTCTCAGAGGATGGGTGTTTCTGAATCTTT
                                                                  

  240 CCTAGTGGCACGTTCATCCCTCACAAATCTCTGCATCTTTCTGACTTTTGTTTTACACAG
                                      >>>>>>>>>>>>>>>>>>>>>>>>>>**

  300 TTGAATATCCATGTGGAAAAATACCTATTCTAGAAAAAAGAAATGCCAGCAAACCCCAAG
      ************************************************************

  360 GCCGAATTGTGGGGGGCAAGGTGTGCCCCAAAGGGGAGTGTCCATGGCAGGTAAGGCTTC
      ****************************************************    <<<<

  420 CCCTGGCTTCAGGATTCCAAGCCCTGAGGGTCTTGAAGCCTTTTGAATGTGAACAACAGC
      <<<<<<<<<<<<<<<                                             

  480 TCTGGAAGGGAAAATGGGCAGGTCAGCCCAAGCCCACAGGCTCCAAGTCAGCACACCTAG
                                                                  

  540 CACCTCCAGCTCGCGGCACCCCCATGCTTTTAGTGGGGCAAGGAAGGAGAAAAGAAAACG
                                                                  

  600 ACACTCACTGAGGGTCTACCCTGTGCAGAGAACCCTGCGAGATGCCCCATCCGAGTTGTC
                                                                  

  660 ACGTCGTCCTCACGGTTACTCTTTGAGGTGGGATCTTTGCCTGATCTTTG
                                                        

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    3891     0   289     0     0    43     0   599  2265     0     0     0    44     0   651
Right   3894     0   289     0     0    11     0   463  2371     0     1     0    44     0   715
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F7_X7

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        262   21   59.64   47.62  4.00  0.00  9.00 AGCAATGTGACTTCCACACCT
POSITION PENALTY, QUALITY: 300.000000, 301.362873
RIGHT PRIMER       447   19   61.08   63.16  2.00  2.00  9.00 CGGACAGGGGAGAGTGGTA
POSITION PENALTY, QUALITY: 60.000000, 62.075168
SEQUENCE SIZE: 724
INCLUDED REGION SIZE: 724

PRODUCT SIZE: 186, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,128

    0 CTTTGCAAAATCAGGAGCATTGGATCAAAGCTATGTGAAGATCCTGTGAGGTGAACAGTG
                                                                  

   60 AAATCTCACAGCGACATTTGTATTCTTGGGCCGTGCCCAAGAGCACGTCTCGGCTAGAGA
                                                                  

  120 GGGGCACAGCCTCCCAGAGCCAGGTCTGAGCAGCTTTGCCTGGGAGGGATCTGCAAAGAC
                                                                  

  180 CCCAGGATTTCAGAAAGAAATTGTGCAATGCCAGAGGTTCCTTGGCATGCCCGGGAGGGC
                                                                  

  240 GAGTCATCAGAGAAACAATGACAGCAATGTGACTTCCACACCTCCTGTCCCCCCGCCCAG
                            >>>>>>>>>>>>>>>>>>>>>               **

  300 GTCCTGTTGTTGGTGAATGGAGCTCAGTTGTGTGGGGGGACCCTGATCAACACCATCTGG
      ************************************************************

  360 GTGGTCTCCGCGGCCCACTGTTTCGACAAAATCAAGAACTGGAGGAACCTGATCGCGGTG
      ************************************************************

  420 CTGGGTGGGTACCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTTCCCT
      ******   <<<<<<<<<<<<<<<<<<<                                

  480 GTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGG
                                                                  

  540 TGCCACTCTCCCCTGTCCGACCGCGGTGCTGGGTGGGTGCCACTCTCCGCTGTCCGACCG
                                                                  

  600 CGGTGCTGGGTGGGTACCACTCTCCCCTGTCTGACCGCAGCTCTCAAGTGTCTCAGGGGC
                                                                  

  660 TGTGGCTCTGGGCTTCGTGCTGTCACTTCCACAGACAGACAGACATCCCCAAAAGGGGAG
                                                                  

  720 CAAC
          

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4032     0   289     0     0     6     0   577  2278     0     0     0    44     0   838
Right   4181     0   289     0     0    10     0   236  3155     0     5     0    22     0   464
Pair Stats:
considered 126, high any compl 28, high end compl 97, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR F7_X8

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        265   18   62.95   66.67  5.00  3.00 10.00 GTGGAAAGGGCCTGAGGG
POSITION PENALTY, QUALITY: 300.000000, 304.949476
RIGHT PRIMER       922   18   59.90   61.11  4.00  3.00  8.00 TTCTCTCCACAGGCCAGG
POSITION PENALTY, QUALITY: 140.000000, 142.096979
SEQUENCE SIZE: 1196
INCLUDED REGION SIZE: 1196

PRODUCT SIZE: 658, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 298,600

    0 GCCGGGAAGAACTGTGGAGAATCGGGGCACGCCCTGTCCTCCCAGCTGCCAGGGCACAGC
                                                                  

   60 ATCCCTTCCCCACCTGCAACACCCAGACCCCAGATTCACCCCAGTTCACTTGTCCCCACA
                                                                  

  120 CGAGCCACAGGCTGCCACCTGGGGCAGGCTGGCCCACCTTGGGGTTAGATGCAGGTCCCC
                                                                  

  180 TTGCCCCAGAAGGAGACTGCAGCCCCTGCAGACCTAGAAATGGCCACAGCCCATCCCCAT
                                                                  

  240 GCACCAGGGGGTGAGGTGGCAGGTGGTGGAAAGGGCCTGAGGGGGGCTTCTTCCTTCCAG
                               >>>>>>>>>>>>>>>>>>               **

  300 GCGAGCACGACCTCAGCGAGCACGACGGGGATGAGCAGAGCCGGCGGGTGGCGCAGGTCA
      ************************************************************

  360 TCATCCCCAGCACGTACGTCCCGGGCACCACCAACCACGACATCGCGCTGCTCCGCCTGC
      ************************************************************

  420 ACCAGCCCGTGGTCCTCACTGACCATGTGGTGCCCCTCTGCCTGCCCGAACGGACGTTCT
      ************************************************************

  480 CTGAGAGGACGCTGGCCTTCGTGCGCTTCTCATTGGTCAGCGGCTGGGGCCAGCTGCTGG
      ************************************************************

  540 ACCGTGGCGCCACGGCCCTGGAGCTCATGGTCCTCAACGTGCCCCGGCTGATGACCCAGG
      ************************************************************

  600 ACTGCCTGCAGCAGTCACGGAAGGTGGGAGACTCCCCAAATATCACGGAGTACATGTTCT
      ************************************************************

  660 GTGCCGGCTACTCGGATGGCAGCAAGGACTCCTGCAAGGGGGACAGTGGAGGCCCACATG
      ************************************************************

  720 CCACCCACTACCGGGGCACGTGGTACCTGACGGGCATCGTCAGCTGGGGCCAGGGCTGCG
      ************************************************************

  780 CAACCGTGGGCCACTTTGGGGTGTACACCAGGGTCTCCCAGTACATCGAGTGGCTGCAAA
      ************************************************************

  840 AGCTCATGCGCTCAGAGCCACGCCCAGGAGTCCTCCTGCGAGCCCCATTTCCCTAGCCCA
      **********************************************************  

  900 GCAGCCCTGGCCTGTGGAGAGAAAGCCAAGGCTGCGTCGAACTGTCCTGGCACCAAATCC
           <<<<<<<<<<<<<<<<<<                                     

  960 CATATATTCTTCTGCAGTTAATGGGGTAGAGGAGGGCATGGGAGGGAGGGAGAGGTGGGG
                                                                  

 1020 AGGGAGACAGAGACAGAAACAGAGAGAGACAGAGACAGAGAGAGACTGAGGGAGAGACTC
                                                                  

 1080 TGAGGACATGGAGAGAGACTCAAAGAGACTCCAAGATTCAAAGAGACTAATAGAGACACA
                                                                  

 1140 GAGATGGAATAGAAAAGATGAGAGGCAGAGGCAGACAGGCGCTGGACAGAGGGGCA
                                                              

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    8901     0   289     0     0    87     0   201  7523     0     0     0    22     0   779
Right   9054     0   289     0     0    79     0  1105  6374     0     1     0     0     0  1206
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HCF2_X2

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   59.94   50.00  1.00  0.00  8.00 TGTGTTCTGTTTTCCCTCCC
POSITION PENALTY, QUALITY: 0.000000, 0.056936
RIGHT PRIMER      1231   23   60.71   47.83  4.00  2.00  9.00 TGTGAGAACTGTAAAGGTGCCTC
POSITION PENALTY, QUALITY: 40.000000, 43.705322
SEQUENCE SIZE: 1505
INCLUDED REGION SIZE: 1505

PRODUCT SIZE: 954, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 298,909

    0 CTCCTCCCTGGGATGCCTGAGCTCCCAGGATTCTAGAAGGTTAGTTTTGCAAACCTTTAA
                                                                  

   60 AGAAGGGATTTTCATCAAGGGGCCCACAGATCCTTCATTGAGGTTTATGAGTCCCACATC
                                                                  

  120 AAAGGTTGGGTGTCTATCTACATCAGATTCTCTTAAAGTCCATGATCCTAAAACAGTTAA
                                                                  

  180 GAACTAATGCTGTGAGGGCCTCTTCCTGGGTCAAAGCCACAGGGAACCTGCCATGTGGAT
                                                                  

  240 GCTGCAGCGGGGTGTGGATCAGCCAGGCCGCCTTTCACTGTGTTCTGTTTTCCCTCCCAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 CTTTAGCTCCGCCAAAATGAAACACTCATTAAACGCACTTCTCATTTTCCTCATCATAAC
      ************************************************************

  360 ATCTGCGTGGGGTGGGAGCAAAGGCCCGCTGGATCAGCTAGAGAAAGGAGGGGAAACTGC
      ************************************************************

  420 TCAGTCTGCAGATCCCCAGTGGGAGCAGTTAAATAACAAAAACCTGAGCATGCCTCTTCT
      ************************************************************

  480 CCCTGCCGACTTCCACAAGGAAAACACCGTCACCAACGACTGGATTCCAGAGGGGGAGGA
      ************************************************************

  540 GGACGACGACTATCTGGACCTGGAGAAGATATTCAGTGAAGACGACGACTACATCGACAT
      ************************************************************

  600 CGTCGACAGTCTGTCAGTTTCCCCGACAGACTCTGATGTGAGTGCTGGGAACATCCTCCA
      ************************************************************

  660 GCTTTTTCATGGCAAGAGCCGGATCCAGCGTCTTAACATCCTCAACGCCAAGTTCGCTTT
      ************************************************************

  720 CAACCTCTACCGAGTGCTGAAAGACCAGGTCAACACTTTCGATAACATCTTCATAGCACC
      ************************************************************

  780 CGTTGGCATTTCTACTGCGATGGGTATGATTTCCTTAGGTCTGAAGGGAGAGACCCATGA
      ************************************************************

  840 ACAAGTGCACTCGATTTTGCATTTTAAAGACTTTGTTAATGCCAGCAGCAAGTATGAAAT
      ************************************************************

  900 CACGACCATTCATAATCTCTTCCGTAAGCTGACTCATCGCCTCTTCAGGAGGAATTTTGG
      ************************************************************

  960 GTACACACTGCGGTCAGTCAATGACCTTTATATCCAGAAGCAGTTTCCAATCCTGCTTGA
      ************************************************************

 1020 CTTCAAAACTAAAGTAAGAGAGTATTACTTTGCTGAGGCCCAGATAGCTGACTTCTCAGA
      ************************************************************

 1080 CCCTGCCTTCATATCAAAAACCAACAACCACATCATGAAGCTCACCAAGGGCCTCATAAA
      ************************************************************

 1140 AGATGCTCTGGAGAATATAGACCCTGCTACCCAGATGATGATTCTCAACTGCATCTACTT
      ************************************************************

 1200 CAAAGGTAAGAGGCACCTTTACAGTTCTCACAGCAAACCCACAACATACTATTTTTGTAT
      *******  <<<<<<<<<<<<<<<<<<<<<<<                            

 1260 GTGGGTAGATTGAATGCCAAGAACTGTACTGTAGCTATAATTTATCCAGGAAAACTAGAC
                                                                  

 1320 ACAAGATTGACTCTGGAACGGGGACAGGGAAGGCCAAGCTGAAGTGACAGTAGCATCTGA
                                                                  

 1380 CACTTACTGAGCCCTAACTCTGTGCTTTAACACAGCCTTGTGAGGTCATCACTGTTATTA
                                                                  

 1440 GCATCCCCATTTTACAGAGGAAGCCACCAACACATGAAGTAAAAGGATGGGCTGGGCGCG
                                                                  

 1500 GTGGC
           

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left   12144     0   289     0     0    12     0  3242  5342     0     0     0     0     0  3259
Right  12144     0   289     0     0    15     0  3651  4687     0     7     0     0     0  3495
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HCF2_X3

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   58.79   55.00  4.00  0.00  8.00 CCTCTTCCTGTGGCCTTTAC
POSITION PENALTY, QUALITY: 0.000000, 1.210543
RIGHT PRIMER       605   23   58.48   39.13  3.00  3.00 10.00 GCTCAAAAGAACAAACACACAGT
POSITION PENALTY, QUALITY: 140.000000, 144.517111
SEQUENCE SIZE: 874
INCLUDED REGION SIZE: 874

PRODUCT SIZE: 328, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 298,278

    0 TTTGACAGATGTGACAGATGAAGAAATAGAAGCGAGTTAGGTGCCTTACCATGGTCAAAC
                                                                  

   60 AACTAGTTCGTATCAGACCCTACTCCAGAAACTATTCCAGTCCGGGTAACCTCTCGTTAA
                                                                  

  120 CCTCTCTTGTTAGAAATGCAAATTTCTGCCCAAATCAGGCCTCAGGAATCAAGAGACTGT
                                                                  

  180 GGGGTCGGCTCTGCAGGCTATCTGAATGAGGCCTCCAGGGAAATCAGATTCACTCTCAAG
                                                                  

  240 GGTGAGACGATTTCCCTAAAGGAACCTTCTCATAACAGCCTCTTCCTGTGGCCTTTACAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 GATCCTGGGTGAATAAATTCCCAGTGGAAATGACACACAACCACAACTTCCGGCTGAATG
      ************************************************************

  360 AGAGAGAGGTAGTTAAGGTTTCCATGATGCAGACCAAGGGGAACTTCCTCGCAGCAAATG
      ************************************************************

  420 ACCAGGAGCTGGACTGCGACATCCTCCAGCTGGAATACGTGGGGGGCATCAGCATGCTAA
      ************************************************************

  480 TTGTGGTCCCACACAAGATGTCTGGGATGAAGACCCTCGAAGCGCAACTGACACCCCGGG
      ************************************************************

  540 TGGTGGAGAGATGGCAAAAAAGCATGACAAACAGGTATTTCACACTGTGTGTTTGTTCTT
      ************************************       <<<<<<<<<<<<<<<<<

  600 TTGAGCTCCCAGATGCTGGGGGTGTCTGGGAATACTGGAAAATGGATCATTTTTTTAAAA
      <<<<<<                                                      

  660 AGGGAGAATTATGTACAAGTACCCAAGAACTTCCATACAGGGCCACTCTGTTAATTCAGC
                                                                  

  720 CCCAATTTGTTGCTTGAGATAAGAGATGATTAGAGAGCATTCATAAGGGACACATCTGCC
                                                                  

  780 CTCTAGGGGCCAGTTTCAGAAGTTAGAGGCAGATGACTTAGAGACAGCTTGGTGCTTGCT
                                                                  

  840 TTGTGGCTTCGAGTCCCAGCTTCATCATCCCTAA
                                        

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    5543     0   289     0     0     0     0  1266  2461     0     0     0    38     0  1489
Right   5393     0   289     0     0    28     0  1331  2335    19     7     0    67     0  1317
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HCF2_X4

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        278   20   59.55   50.00  1.00  0.00  9.00 CCCCCTTTCCTTTTCTGTCT
POSITION PENALTY, QUALITY: 0.000000, 0.451656
RIGHT PRIMER       465   19   59.80   57.89  2.00  0.00  8.00 GGTGGACAAGGGAGTGGTT
POSITION PENALTY, QUALITY: 0.000000, 1.198986
SEQUENCE SIZE: 745
INCLUDED REGION SIZE: 745

PRODUCT SIZE: 188, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,149

    0 ATGCCTTTTTAAACCATAAACCAGGAAAAGTTCTGCTGCTAACCTTGAAGATAGGAAACG
                                                                  

   60 AACCATACAGTCTCAAGGAAATAATCATGCAACAGAAAACACACCTCAGTTTTCAGTAGC
                                                                  

  120 GGAATTACAAAGGAGTGTGCTTCCTAAAATCCTCAACTGACAGTCCCGGAATATAAATTT
                                                                  

  180 TAATAAGTGCTATATCAATTCTGTGATAAATATAACCCGTGGCCCTTTAAAGGGAAAATC
                                                                  

  240 ATGATTCTTTTGTAACTTGTGGTTCAATAAAACTGGGCCCCCCTTTCCTTTTCTGTCTAG
                                            >>>>>>>>>>>>>>>>>>>>**

  300 AACTCGAGAAGTGCTTCTGCCGAAATTCAAGCTGGAGAAGAACTACAATCTAGTGGAGTC
      ************************************************************

  360 CCTGAAGTTGATGGGGATCAGGATGCTGTTTGACAAAAATGGCAACATGGCAGGCATCTC
      ************************************************************

  420 AGACCAAAGGATCGCCATCGACCTGGTAACCACTCCCTTGTCCACCCCCGACCCGTCCCC
      ***************************<<<<<<<<<<<<<<<<<<<              

  480 AGGGTCTGCCTCAGCACAGCCCCACCTCCACTTGCCCTTCCTACCCACCCCCCAATCTCA
                                                                  

  540 TGTCCCAGCTTGGGGTGCTGAGTCTGCTCTTCGGCCTGGGTGGGATACACAGAATGCCTA
                                                                  

  600 GTTTCATGGATGCCAGCTGGAGAGCACGGCACCTGGCAGACACTTACTGGGCAGGGGGGA
                                                                  

  660 TCCCAAGAGCAGCCATGGGGTGAGCCCCACTCCCGCTGACACCAGAGACAGGGGAGACAT
                                                                  

  720 GTGCTGCGGTCTGGGAAATAGCTAC
                               

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    4391     0   289     0     0   128     0  1570  1096     0     0     0    22     0  1286
Right   4238     0   289     0     0    20     0   420  2770     0     0     0    44     0   695
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HCF2_X5

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER        275   23   59.66   39.13  4.00  2.00 10.00 TCTGACAACTTTCCTTTCCAAAC
POSITION PENALTY, QUALITY: 0.000000, 3.339047
RIGHT PRIMER      1193   23   59.76   34.78  4.00  2.00 10.00 TTGGCTTTTGATATTTGAGCTTC
POSITION PENALTY, QUALITY: 460.000000, 463.239398
SEQUENCE SIZE: 1446
INCLUDED REGION SIZE: 1446

PRODUCT SIZE: 919, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 298,850

    0 CCCGGAGAAGTGCGCAGCAGTGTGGGGAGCTGGAGCTGGGGTGGCTGTCCTGCACCAGCC
                                                                  

   60 CCCACGACCCTCAGACCACAGGCACTGCCAAGAGGGAACATGAACCTAGCCGGCCTCTAA
                                                                  

  120 GTGCAACGGCTGCCCCTGACAGGTGGTGACAGATATTTTCAAGAGTGACTCTGACCAGCT
                                                                  

  180 GTGATTTCCACCTTACATGTTGTCTTTGGATCCTTTCCCTGAATGATATGAGATTGTGCT
                                                                  

  240 GGGAACTCTAGCCCTCTGTGTGCTGACCTCCAGAATCTGACAACTTTCCTTTCCAAACAG
                                         >>>>>>>>>>>>>>>>>>>>>>>**

  300 TTCAAGCACCAAGGCACGATCACAGTGAACGAGGAAGGCACCCAAGCCACCACTGTGACC
      ************************************************************

  360 ACGGTGGGGTTCATGCCGCTGTCCACCCAAGTCCGCTTCACTGTCGACCGCCCCTTTCTT
      ************************************************************

  420 TTCCTCATCTACGAGCATCGCACCAGCTGCCTGCTCTTCATGGGAAGAGTGGCCAACCCC
      ************************************************************

  480 AGCAGGTCCTAGAGGTGGAGGTCTAGGTGTCTGAAGTGCCTTGGGGGCACCCTCATTTTG
      ************************************************************

  540 TTTCCATTCCAACAACGAGAACAGAGATGTTCTGGCATCATTTACGTAGTTTACGCTACC
      ************************************************************

  600 AATCTGAATTCGAGGCCCATATGAGAGGAGCTTAGAAACGACCAAGAAGAGAGGCTTGTT
      ************************************************************

  660 GGAATCAATTCTGCACAATAGCCCATGCTGTAAGCTCATAGAAGTCACTGTAACTGTAGT
      ************************************************************

  720 GTGTCTGCTGTTACCTAGAGGGTCTCACCTCCCCACTCTTCACAGCAAACCTGAGCAGCG
      ************************************************************

  780 CGTCCTAAGCACCTCCCGCTCCGGTGACCCCATCCTTGCACACCTGACTCTGTCACTCAA
      ************************************************************

  840 GCCTTTCTCCACCAGGCCCCTCATCTGAATACCAAGCACAGAAATGAGTGGTGTGACTAA
      ************************************************************

  900 TTCCTTACCTCTCCCAAGGAGGGTACACAACTAGCACCATTCTTGATGTCCAGGGAAGAA
      ************************************************************

  960 GCCACCTCAAGACATATGAGGGGTGCCCTGGGCTAATGTTAGGGCTTAATTTTCTCAAAG
      ************************************************************

 1020 CCTGACCTTTCAAATCCATGATGAATGCCATCAGTCCCTCCTGCTGTTGCCTCCCTGTGA
      ************************************************************

 1080 CCTGGAGGACAGTGTGTGCCATGTCTCCCATACTAGAGATAAATAAATGTAGCCACATTT
      ************************************************************

 1140 ACTGTGTATCTGTTATAATTCTCTATTTTTTGAAGCTCAAATATCAAAAGCCAAATCCAA
      ********                       <<<<<<<<<<<<<<<<<<<<<<<      

 1200 ATTCCTGGATAACTCCAGGTATGATAAAGGCTGAGAGGAAGTCACTTGAGCACCACAATG
                                                                  

 1260 TGCCACAGCAGGGCATGTTCTCAGGACAGGACAGGTGTGTGCTGAATCCTGGGGAGGGTC
                                                                  

 1320 TGTGCAGTACCCCAGAACTGTGGGGTGCTAAGTGGCACACAAGCCCCAGGGCTCCCACAG
                                                                  

 1380 TCTATGCCAGGCTGCTGCAGCTTTCATCCCTCATACCTGGTCCTGCAGTGGGTCTGGTTT
                                                                  

 1440 GACAGA
            

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left   11554     0   289     0     0     7     0  2490  6121     0     0     0     0     0  2647
Right  11432     0   289     0     0    57     0  2518  5947     0    12     0    20     0  2589
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X1

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER         39   20   57.72   50.00  6.00  3.00  8.00 TGAGATCTGGAGGATCCAAG
POSITION PENALTY, QUALITY: 300.000000, 302.282278
RIGHT PRIMER       237   24   57.87   29.17  7.00  2.00  9.00 TCTTCAAACTTTGGACATTCATAA
POSITION PENALTY, QUALITY: 24000.000000, 24006.125439
SEQUENCE SIZE: 238
INCLUDED REGION SIZE: 238

PRODUCT SIZE: 199, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 74,164

    0 CCAGGCTGAGCGTCGGCGCCGGGGTTCGGTGGCCTCTAGTGAGATCTGGAGGATCCAAGG
                                             >>>>>>>>>>>>>>>>>>>> 

   60 ATTCTGTAGCTACAATGTTGTCAAGACTTTTTCGAATGCATGGCCTCTTTGTGGCCTCCC
                    **********************************************

  120 ATCCCTGGGAAGTCATAGTGGGGACAGTGACACTGACCATCTGCATGATGTCCATGAACA
      ************************************************************

  180 TGTTTACTGGTAACAATAAGATCTGTGGTTGGAATTATGAATGTCCAAAGTTTGAAGA
      **********************************((((((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    2030     0     0     0     0    67     0   611   842     3    38     0     0     0   469
Right   1490     0    65     0     0     0     0   478   584     0     0     0     0     0   363
Pair Stats:
considered 11, high end compl 10, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X2

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   20   57.20   45.00  3.00  3.00 10.00 GGATGTTTTGAGCAGTGACA
POSITION PENALTY, QUALITY: 20000.000000, 20002.797253
RIGHT PRIMER       108   26   57.38   26.92  8.00  2.00  9.00 AATATATTTTGATCCAAGTTGACGTA
POSITION PENALTY, QUALITY: 29000.000000, 29008.623090
SEQUENCE SIZE: 112
INCLUDED REGION SIZE: 112

PRODUCT SIZE: 109, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 0,112

    0 GGATGTTTTGAGCAGTGACATTATAATTCTGACAATAACACGATGCATAGCCATCCTGTA
      ))))))))))))))))))))****************************************

   60 TATTTACTTCCAGTTCCAGAATTTACGTCAACTTGGATCAAAATATATTTTG
      ***********************((((((((((((((((((((((((((***

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     770     0     0     0     0     5     0   460    81     0     0     0     0     0   224
Right    770     0     0     0     0    11     0   448    81     7     1     0     0     0   222
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X3

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   19   58.71   47.37  4.00  1.00  9.00 GGTATTGCTGGCCTTTTCA
POSITION PENALTY, QUALITY: 19000.000000, 19002.290742
RIGHT PRIMER        87   21   57.47   38.10  4.00  0.00  9.00 TCAAGCCTGTCAATTCTTTGT
POSITION PENALTY, QUALITY: 21000.000000, 21003.532432
SEQUENCE SIZE: 88
INCLUDED REGION SIZE: 88

PRODUCT SIZE: 88, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 0,88

    0 GGTATTGCTGGCCTTTTCACAATTTTCTCAAGTTTTGTATTCAGTACAGTTGTCATTCAC
      )))))))))))))))))))*****************************************

   60 TTCTTAGACAAAGAATTGACAGGCTTGA
      *******(((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     530     0     0     0     0     1     0   361    44     0     0     0     0     0   124
Right    530     0     0     0     0     1     0   416     4     0     0     0     0     0   109
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X4

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   20   58.44   40.00  6.00  0.00 10.00 ATGAAGCTTTGCCCTTTTTC
POSITION PENALTY, QUALITY: 20000.000000, 20001.557050
RIGHT PRIMER        84   19   57.08   52.63  6.00  0.00  8.00 TGTGAGTTGGAACTGAGGG
POSITION PENALTY, QUALITY: 19000.000000, 19003.924962
SEQUENCE SIZE: 85
INCLUDED REGION SIZE: 85

PRODUCT SIZE: 85, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 0,85

    0 ATGAAGCTTTGCCCTTTTTCCTACTTTTGATTGACCTTTCCAGAGCAAGCACATTAGCAA
      ))))))))))))))))))))****************************************

   60 AGTTTGCCCTCAGTTCCAACTCACA
      ******(((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     500     0     0     0     0     0     0   151   175     0     0     0     0     0   174
Right    500     0     0     0     0     0     0   123   202     0     0     0     0     0   175
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X5

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   25   60.11   36.00  6.00  2.00 10.00 GGATGAAGTAAGGGAAAATATTGCT
POSITION PENALTY, QUALITY: 25000.000000, 25005.106680
RIGHT PRIMER       105   22   59.72   45.45  8.00  0.00 11.00 TGACATGGTACCAACTCCAATC
POSITION PENALTY, QUALITY: 22000.000000, 22002.282868
SEQUENCE SIZE: 106
INCLUDED REGION SIZE: 106

PRODUCT SIZE: 106, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 0,106

    0 GGATGAAGTAAGGGAAAATATTGCTCGTGGAATGGCAATTTTAGGTCCTACGTTTACCCT
      )))))))))))))))))))))))))***********************************

   60 CGATGCTCTTGTTGAATGTCTTGTGATTGGAGTTGGTACCATGTCA
      ************************((((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     710     0     0     0     0     0     0   212   246     0     1     0     0     0   251
Right    710     0     0     0     0     0     0   202   248     0     1     1     0     0   258
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X6

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   18   57.14   55.56  4.00  3.00 10.00 GGGGTACGTCAGCTTGAA
POSITION PENALTY, QUALITY: 18000.000000, 18004.862764
RIGHT PRIMER       106   23   58.33   43.48  3.00  2.00 11.00 TCTAATACCAAGGACACACAAGC
POSITION PENALTY, QUALITY: 23000.000000, 23004.673725
SEQUENCE SIZE: 107
INCLUDED REGION SIZE: 107

PRODUCT SIZE: 107, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 0,107

    0 GGGGTACGTCAGCTTGAAATTATGTGCTGCTTTGGCTGCATGTCAGTTCTTGCCAACTAC
      ))))))))))))))))))******************************************

   60 TTCGTGTTCATGACTTTCTTCCCAGCTTGTGTGTCCTTGGTATTAGA
      ************************(((((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     720     0     0     0     0     0     0   206   309     0     0     0     0     0   205
Right    720     0     0     0     0     0     0   181   338     0     0     0     0     0   201
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X7

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER         17   18   61.02   55.56  4.00  3.00  9.00 GCGAGGGTCGTCCAATTT
POSITION PENALTY, QUALITY: 35000.000000, 35003.015981
RIGHT PRIMER       116   24   57.39   37.50  5.00  1.00  9.00 ATAATCATCTTGACCCTCTGAGTT
POSITION PENALTY, QUALITY: 24000.000000, 24006.612999
SEQUENCE SIZE: 117
INCLUDED REGION SIZE: 117

PRODUCT SIZE: 100, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 0,117

    0 GCTTTCTCGGGAAAGCCGCGAGGGTCGTCCAATTTGGCAGCTCAGCCATTTTGCCCGAGT
      *****************))))))))))))))))))*************************

   60 TTTAGAAGAAGAAGAAAATAAGCCGAATCCTGTAACTCAGAGGGTCAAGATGATTAT
      *********************************((((((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     820     0     0     0     0     3     0   162   464     0     0     0     0     0   191
Right    820     0     0     0     0     3     0   237   349     0     0     0     0     0   231
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X8

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          1   22   57.75   45.45  4.00  0.00 10.00 TCTCTAGGCTTGGTTCTTGTTC
POSITION PENALTY, QUALITY: 23000.000000, 23004.249004
RIGHT PRIMER       154   22   59.14   45.45  5.00  0.00 11.00 GATAAAACTGCCAGAGGGAAAC
POSITION PENALTY, QUALITY: 28000.000000, 28002.863756
SEQUENCE SIZE: 161
INCLUDED REGION SIZE: 161

PRODUCT SIZE: 154, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 0,161

    0 GTCTCTAGGCTTGGTTCTTGTTCATGCTCACAGTCGCTGGATAGCTGATCCTTCTCCTCA
      *))))))))))))))))))))))*************************************

   60 AAACAGTACAGCAGATACTTCTAAGGTTTCATTAGGACTGGATGAAAATGTGTCCAAGAG
      ************************************************************

  120 AATTGAACCAAGTGTTTCCCTCTGGCAGTTTTATCTCTCTA
      *************((((((((((((((((((((((******

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    1260     0     0     0     0     0     0   540   321     0     0     0     0     0   399
Right   1260     0     0     0     0     0     0   530   315     0     0    20     0     0   395
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X9

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   23   58.46   30.43  6.00  3.00 11.00 AAATGATCAGCATGGATATTGAA
POSITION PENALTY, QUALITY: 23000.000000, 23004.538081
RIGHT PRIMER       247   21   57.37   38.10  3.00  2.00 10.00 TTTTCTTTCTCGGTTTATCCC
POSITION PENALTY, QUALITY: 21000.000000, 21003.627403
SEQUENCE SIZE: 248
INCLUDED REGION SIZE: 248

PRODUCT SIZE: 248, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 0,248

    0 AAATGATCAGCATGGATATTGAACAAGTTATTACCCTAAGTTTAGCTCTCCTTCTGGCTG
      )))))))))))))))))))))))*************************************

   60 TCAAGTACATCTTCTTTGAACAAACAGAGACAGAATCTACACTCTCATTAAAAAACCCTA
      ************************************************************

  120 TCACATCTCCTGTAGTGACACAAAAGAAAGTCCCAGACAATTGTTGTAGACGTGAACCTA
      ************************************************************

  180 TGCTGGTCAGAAATAACCAGAAATGTGATTCAGTAGAGGAAGAGACAGGGATAAACCGAG
      ***********************************************(((((((((((((

  240 AAAGAAAA
      ((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    1977     0     0     0     0     1     0  1154   176     0     1     0    22     0   623
Right   1977     0     0     0     0     0     0  1125   203     0     0     0    22     0   627
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X10

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          1   24   57.83   37.50  8.00  3.00  9.00 TTGAGGTTATAAAACCCTTAGTGG
POSITION PENALTY, QUALITY: 25000.000000, 25006.169796
RIGHT PRIMER        89   24   57.71   41.67  4.00  0.00  9.00 TGAAGTATCGAGTAAGGAGGAGTT
POSITION PENALTY, QUALITY: 24000.000000, 24006.288343
SEQUENCE SIZE: 90
INCLUDED REGION SIZE: 90

PRODUCT SIZE: 89, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 0,90

    0 GTTGAGGTTATAAAACCCTTAGTGGCTGAAACAGATACCCCAAACAGAGCTACATTTGTG
      *))))))))))))))))))))))))***********************************

   60 GTTGGTAACTCCTCCTTACTCGATACTTCA
      ******((((((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     550     0     0     0     0     0     0   222   109     0     0     0     0     0   219
Right    550     0     0     0     0     0     0   227   116     0     0     0     0     0   207
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X11

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          1   22   59.54   54.55  8.00  3.00 10.00 CAGTACTGGTGACACAGGAACC
POSITION PENALTY, QUALITY: 23000.000000, 23002.457721
RIGHT PRIMER        88   21   57.28   42.86  4.00  0.00  8.00 TCTGCATTCCCAAGTATCTGT
POSITION PENALTY, QUALITY: 21000.000000, 21003.718624
SEQUENCE SIZE: 89
INCLUDED REGION SIZE: 89

PRODUCT SIZE: 88, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 0,89

    0 TCAGTACTGGTGACACAGGAACCTGAAATTGAACTTCCCAGGGAACCTCGGCCTAATGAA
      *))))))))))))))))))))))*************************************

   60 GAATGTCTACAGATACTTGGGAATGCAGA
      ********(((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     540     0     0     0     0     0     0   135   249     0     1     0     0     0   155
Right    540     0     0     0     0     0     0   181   215     0     0     0     0     0   144
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X12

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   23   59.21   39.13  4.00  1.00  9.00 GAAAGGTGCAAAATTCCTTAGTG
POSITION PENALTY, QUALITY: 23000.000000, 23003.785045
RIGHT PRIMER       194   27   57.50   33.33  8.00  2.00  9.00 AAGGAGTAATTATAATCCCTGTAAGGT
POSITION PENALTY, QUALITY: 27000.000000, 27009.501708
SEQUENCE SIZE: 195
INCLUDED REGION SIZE: 195

PRODUCT SIZE: 195, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 0,195

    0 GAAAGGTGCAAAATTCCTTAGTGATGCTGAGATCATCCAGTTAGTCAATGCTAAGCATAT
      )))))))))))))))))))))))*************************************

   60 CCCAGCCTACAAGTTGGAAACTCTGATGGAAACTCATGAGCGTGGTGTATCTATTCGCCG
      ************************************************************

  120 ACAGTTACTTTCCAAGAAGCTTTCAGAACCTTCTTCTCTCCAGTACCTACCTTACAGGGA
      ************************************************((((((((((((

  180 TTATAATTACTCCTT
      (((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    1600     0     0     0     0     0     0   646   347     0     0     0     0     0   607
Right   1600     0     0     0     0     0     0   707   324     0     0     0     0     0   569
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X13

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   18   57.47   55.56  4.00  0.00  9.00 GGTGATGGGAGCTTGTTG
POSITION PENALTY, QUALITY: 18000.000000, 18004.525596
RIGHT PRIMER       158   19   58.71   57.89  6.00  1.00 10.00 CCTATTGCTCTGCAGCCTC
POSITION PENALTY, QUALITY: 19000.000000, 19002.288404
SEQUENCE SIZE: 159
INCLUDED REGION SIZE: 159

PRODUCT SIZE: 159, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 0,159

    0 GGTGATGGGAGCTTGTTGTGAGAATGTTATTGGATATATGCCCATCCCTGTTGGAGTGGC
      ))))))))))))))))))******************************************

   60 AGGACCCCTTTGCTTAGATGAAAAAGAATTTCAGGTTCCAATGGCAACAACAGAAGGTTG
      ************************************************************

  120 TCTTGTGGCCAGCACCAATAGAGGCTGCAGAGCAATAGG
      ********************(((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    1240     0     0     0     0     1     0   314   591     0     0     0     0     0   334
Right   1240     0     0     0     0     1     0   280   628     0     0     0     0     0   331
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X14

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   18   60.84   61.11  3.00  1.00  9.00 TCTTGGTGGAGGTGCCAG
POSITION PENALTY, QUALITY: 18000.000000, 18002.835611
RIGHT PRIMER       157   20   60.02   50.00  4.00  2.00  9.00 TGCTAGTGCTGTCAAATGCC
POSITION PENALTY, QUALITY: 20000.000000, 20000.018700
SEQUENCE SIZE: 158
INCLUDED REGION SIZE: 158

PRODUCT SIZE: 158, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 0,158

    0 TCTTGGTGGAGGTGCCAGCAGCCGAGTCCTTGCAGATGGGATGACTCGTGGCCCAGTTGT
      ))))))))))))))))))******************************************

   60 GCGTCTTCCACGTGCTTGTGACTCTGCAGAAGTGAAAGCCTGGCTCGAAACATCTGAAGG
      ************************************************************

  120 GTTCGCAGTGATAAAGGAGGCATTTGACAGCACTAGCA
      ******************((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    1230     0     0     0     0     0     0    83   898     0     0     0     0     0   249
Right   1230     0     0     0     0     0     0   122   805     0     0     0     0     0   303
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X15

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   23   57.64   39.13  4.00  3.00 11.00 GATTTGCACGTCTACAGAAACTT
POSITION PENALTY, QUALITY: 23000.000000, 23005.359396
RIGHT PRIMER       105   21   59.61   38.10  7.00  0.00  9.00 TTTGAAATCATGTTCATCCCC
POSITION PENALTY, QUALITY: 21000.000000, 21001.386646
SEQUENCE SIZE: 106
INCLUDED REGION SIZE: 106

PRODUCT SIZE: 106, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 0,106

    0 GATTTGCACGTCTACAGAAACTTCATACAAGTATAGCTGGACGCAACCTTTATATCCGTT
      )))))))))))))))))))))))*************************************

   60 TCCAGTCCAGGTCAGGGGATGCCATGGGGATGAACATGATTTCAAA
      *************************(((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     710     0     0     0     0     0     0   257   250     0     0     0     0     0   203
Right    710     0     0     0     0     0     0   172   343     0     0     0     0     0   195
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X16

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          5   23   57.09   34.78  8.00  3.00 11.00 CAGAGAAAGCACTTTCAAAACTT
POSITION PENALTY, QUALITY: 28000.000000, 28005.907183
RIGHT PRIMER       170   20   61.39   55.00  4.00  2.00  9.00 TCTCTGACAACCTTGGCTGG
POSITION PENALTY, QUALITY: 20000.000000, 20001.389940
SEQUENCE SIZE: 171
INCLUDED REGION SIZE: 171

PRODUCT SIZE: 166, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 0,171

    0 GGGTACAGAGAAAGCACTTTCAAAACTTCACGAGTATTTCCCTGAAATGCAGATTCTAGC
      *****)))))))))))))))))))))))********************************

   60 CGTTAGTGGTAACTATTGTACTGACAAGAAACCTGCTGCTATAAATTGGATAGAGGGAAG
      ************************************************************

  120 AGGAAAATCTGTTGTTTGTGAAGCTGTCATTCCAGCCAAGGTTGTCAGAGA
      *******************************((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    1360     0     0     0     0     0     0   684   181     0     8     2     0     0   485
Right   1360     0     0     0     0     0     0   616   248     0     1     0     0     0   495
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X17

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          6   23   57.22   43.48  5.00  3.00 10.00 AAAGACTACCACAGAGGCTATGA
POSITION PENALTY, QUALITY: 29000.000000, 29005.780871
RIGHT PRIMER       140   21   59.58   47.62  4.00  2.00  9.00 TGTCCACAGGCAATGTAGATG
POSITION PENALTY, QUALITY: 21000.000000, 21001.418918
SEQUENCE SIZE: 141
INCLUDED REGION SIZE: 141

PRODUCT SIZE: 135, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 2.00
TARGETS (start, len)*: 0,141

    0 AGTATTAAAGACTACCACAGAGGCTATGATTGAGGTCAACATTAACAAGAATTTAGTGGG
      ******)))))))))))))))))))))))*******************************

   60 CTCTGCCATGGCTGGGAGCATAGGAGGCTACAACGCCCATGCAGCAAACATTGTCACCGC
      ************************************************************

  120 CATCTACATTGCCTGTGGACA
      (((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    1060     0     0     0     0     1     0   295   528     0     3     0     0     0   233
Right   1060     0     0     0     0     1     0   217   601     0     0     0     0     0   241
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X18

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   20   60.27   50.00  5.00  2.00  9.00 GGATGCAGCACAGAATGTTG
POSITION PENALTY, QUALITY: 20000.000000, 20000.272067
RIGHT PRIMER       157   19   62.07   57.89  6.00  1.00  9.00 GCAAACAGGCTTGCTGAGG
POSITION PENALTY, QUALITY: 20000.000000, 20003.072251
SEQUENCE SIZE: 159
INCLUDED REGION SIZE: 159

PRODUCT SIZE: 158, PAIR ANY COMPL: 7.00, PAIR 3' COMPL: 3.00
TARGETS (start, len)*: 0,159

    0 GGATGCAGCACAGAATGTTGGTAGTTCAAACTGTATTACTTTAATGGAAGCAAGTGGTCC
      ))))))))))))))))))))****************************************

   60 CACAAATGAAGATTTATATATCAGCTGCACCATGCCATCTATAGAGATAGGAACGGTGGG
      ************************************************************

  120 TGGTGGGACCAACCTACTACCTCAGCAAGCCTGTTTGCA
      *******************(((((((((((((((((((*

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    1240     0     0     0     0    18     0   522   395     0     0     2     0     0   303
Right   1240     0     0     0     0    18     0   504   411     0     0     0     0     0   307
Pair Stats:
considered 62, high end compl 59, ok 3
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X19

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   21   58.95   52.38  4.00  2.00  9.00 GATGCTAGGTGTTCAAGGAGC
POSITION PENALTY, QUALITY: 21000.000000, 21002.049610
RIGHT PRIMER       154   23   58.59   34.78  5.00  0.00 11.00 TGTTGTGAATCATGTGACTTTTG
POSITION PENALTY, QUALITY: 23000.000000, 23004.407655
SEQUENCE SIZE: 155
INCLUDED REGION SIZE: 155

PRODUCT SIZE: 155, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len)*: 0,155

    0 GATGCTAGGTGTTCAAGGAGCATGCAAAGATAATCCTGGGGAAAATGCCCGGCAGCTTGC
      )))))))))))))))))))))***************************************

   60 CCGAATTGTGTGTGGGACCGTAATGGCTGGGGAATTGTCACTTATGGCAGCATTGGCAGC
      ************************************************************

  120 AGGACATCTTGTCAAAAGTCACATGATTCACAACA
      ************(((((((((((((((((((((((

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left    1200     0     0     0     0     0     0   119   823     0     0     0     0     0   258
Right   1200     0     0     0     0     0     0   167   784     0     0     0     0     0   249
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR HMGCR_X20

Using mispriming library not_for_production.lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   24   60.24   41.67  4.00  2.00  9.00 GGTCGAAGATCAATTTACAAGACC
POSITION PENALTY, QUALITY: 24000.000000, 24004.237346
RIGHT PRIMER        87   19   59.62   52.63  4.00  3.00  8.00 GCCCATGTTCCAGTTCAGA
POSITION PENALTY, QUALITY: 260.000000, 261.379041
SEQUENCE SIZE: 164
INCLUDED REGION SIZE: 164

PRODUCT SIZE: 88, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len)*: 0,56

    0 GGTCGAAGATCAATTTACAAGACCTCCAAGGAGCTTGCACCAAGAAGACAGCCTGAATAG
      ))))))))))))))))))))))))********************************    

   60 CCCGACAGTTCTGAACTGGAACATGGGCATTGGGTTCTAAAGGACTAACATAAAATCTGT
               <<<<<<<<<<<<<<<<<<<                                

  120 GAATTAAAAAAGCTCAATGCATTGTCTTGTGGAGGATGAATAAA
                                                  

KEYS (in order of precedence):
****** target
>>>>>> left primer
<<<<<< right primer


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left     444     0    99     0     0     0     0    91   152     0     0     0     0     0   102
Right   1150     0     0     0     0    42     0   311   512    26     3     0    20     0   236
Pair Stats:
considered 1, ok 1
primer3 release 2.0.0


