SEQUENCE_ID=Load qPCR settings
SEQUENCE_TEMPLATE=atggatttgatggacatggatgccatcaatgacctccacttctctggacacagctccctcaccaacacccctacttccgacgaggattatggttgcacctggaaccactggtctcccatcgtcaactgggacacctttacaggtgccccagatgactttcaccacctcatggacaccatcatcgaggacagaacaactgtcctcgaacagctcagtccatctatcaccacaaccaccaccaccaccacaaccacggatgaggaagaagaagaaatggaaacaacaaccacaacaacaacaacggcaataaaaacgcatgaagttggtgatgattcaaaagggctaaaactagtgcacctattgatggctggcgcggaagccttaaccggttcaaccaaaaaccgtgacttagctcgagtgatattgatccggctcaaggaattagtctcacaacatgctaacggctccaacatggagagacttgccgctcatttcaccgaagcccttcatggactactagaaggtgctgggggtgcgcataataaccaccatcaccacaacaacaacaaacattacctcaccacaaatggtccccacgacaaccaaaacgacacacttgctgctttccaactacttcaagacatgtcaccttacgtcaaattcggccacttcacagccaatcaagccatcatcgaagccgtggcccacgaacgccgcgtccatgtcatcgactacgatatcatggaaggggtccaatgggcctcattaatccaatcacttgcttccaacaacaacggtccacatcttcggataaccgcattatcgcggacaggaaccggccggaggtcaatcgccaccgtacaagaaaccggcaggcgattaacctcctttgccgcttccctcggacaaccattttcttttcatcactgcagattagactccgatgaaactttccgtccttctgcattaaagcttgtacgtggagaggctttggttttcaactgcatgctgaatttacctcaccttagttaccgtgcaccggaatcagttgcttcgtttttaaacggagcaaaaacgttgaatccaaagcttgtgacgttggttgaagaagaagttggttctgttattggtgggtttgtggaaaggttcatggactcacttcatcattattcagcggtttttgattcattggaggctggttttccgatgcagaaccgggcccggactttggtggagagggtattttttgggcctagaattgcaggctcgttgggccggatatatagaacgggtggtgaagaggagagaaggtcatggggggagtggttaggtgaggtagggtttaggggagttccggtgagctttgcaaatcattgtcaagcaaagctgttgttagggctttttaatgatgggtatagggttgaagaggtgggtgtgggtagtaataagttggtgttggattggaaatcaagacgtttgctttctgcttccctttggacttgttcttcttcagattctgatttatag
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=5
PRIMER_TASK=pick_detection_primers
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.013026
PRIMER_LEFT_0_PENALTY=0.006937
PRIMER_RIGHT_0_PENALTY=0.006089
PRIMER_LEFT_0_SEQUENCE=gtcctcgaacagctcagtcc
PRIMER_RIGHT_0_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_0=198,20
PRIMER_RIGHT_0=315,20
PRIMER_LEFT_0_TM=59.993
PRIMER_RIGHT_0_TM=60.006
PRIMER_LEFT_0_GC_PERCENT=60.000
PRIMER_RIGHT_0_GC_PERCENT=40.000
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=2.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=7.6000
PRIMER_RIGHT_0_END_STABILITY=7.0000
PRIMER_PAIR_0_COMPL_ANY=4.00
PRIMER_PAIR_0_COMPL_END=1.00
PRIMER_PAIR_0_PRODUCT_SIZE=118
PRIMER_PAIR_0_PRODUCT_TM=74.3
PRIMER_PAIR_1_PENALTY=0.020508
PRIMER_LEFT_1_PENALTY=0.014419
PRIMER_RIGHT_1_PENALTY=0.006089
PRIMER_LEFT_1_SEQUENCE=ttctctggacacagctccct
PRIMER_RIGHT_1_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_1=39,20
PRIMER_RIGHT_1=315,20
PRIMER_LEFT_1_TM=59.986
PRIMER_RIGHT_1_TM=60.006
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=277
PRIMER_PAIR_1_PRODUCT_TM=78.6
PRIMER_PAIR_2_PENALTY=0.020508
PRIMER_LEFT_2_PENALTY=0.014419
PRIMER_RIGHT_2_PENALTY=0.006089
PRIMER_LEFT_2_SEQUENCE=cttctctggacacagctccc
PRIMER_RIGHT_2_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_2=38,20
PRIMER_RIGHT_2=315,20
PRIMER_LEFT_2_TM=59.986
PRIMER_RIGHT_2_TM=60.006
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=278
PRIMER_PAIR_2_PRODUCT_TM=78.7
PRIMER_PAIR_3_PENALTY=0.032388
PRIMER_LEFT_3_PENALTY=0.032351
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_LEFT_3_SEQUENCE=gacacacttgctgctttcca
PRIMER_RIGHT_3_SEQUENCE=gtttcttgtacggtggcgat
PRIMER_LEFT_3=609,20
PRIMER_RIGHT_3=868,20
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=260
PRIMER_PAIR_3_PRODUCT_TM=78.9
PRIMER_PAIR_4_PENALTY=0.037132
PRIMER_LEFT_4_PENALTY=0.031043
PRIMER_RIGHT_4_PENALTY=0.006089
PRIMER_LEFT_4_SEQUENCE=actggtctcccatcgtcaac
PRIMER_RIGHT_4_SEQUENCE=gcgtttttattgccgttgtt
PRIMER_LEFT_4=106,20
PRIMER_RIGHT_4=315,20
PRIMER_LEFT_4_TM=59.969
PRIMER_RIGHT_4_TM=60.006
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PAIR_4_PRODUCT_SIZE=210
PRIMER_PAIR_4_PRODUCT_TM=77.4
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